Protective effects of farrerol against hydrogen-peroxide-induced apoptosis in human endothelium-derived EA.hy926 cells.

Abstract

Vascular endothelium plays an important role in the physiological homeostasis of blood vessels. Endothelial injury is considered to be implicated in the pathogenesis of many cardiovascular diseases, including atherosclerosis. Farrerol, a flavonoid considered to be the major bioactive component in a traditional Chinese herb, "Man-shan-hong", which is the dried leaves of Rhododendron dauricum L., displays many bioactive properties, including antibechic, antibacterial, anti-inflammatory, and the inhibition of vascular smooth muscle cell (VSMC) proliferation. In this study, the protective effects of farrerol on hydrogen peroxide (H2O2)-induced apoptosis in human endothelium-derived EA.hy926 cells were investigated. The results showed that farrerol significantly inhibited the loss of cell viability and enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in H2O2-induced EA.hy926 cells. Meanwhile, farrerol inhibited H2O2-induced elevation in the levels of intracellular malondialdehyde and reactive oxygen species, as well as cell apoptosis. Furthermore, real time RT-PCR and Western blot analysis showed that farrerol significantly decreased the expression of Bax mRNA, Bax, cleaved caspase-3, and phosph-p38 MAPK, while increasing the exporession of Bcl-2 mRNA and Bcl-2 in H2O2-induced EA.hy926 cells. These results are the first demonstration that farrerol has protective effects against H2O2-induced apoptosis in EA.hy926 cells, and suggests that farrerol is a potential candidate for the intervention of endothelial-injury-associated cardiovascular diseases.

• Authors: Jian-Kuan Li, Rui Ge, Li Tang, Qing-Shan Li
• Journal: Canadian journal of physiology and pharmacology (Can J Physiol Pharmacol) Vol. 91 Issue 9 Pg. 733-40 (Sep 2013) ISSN: 1205-7541 [Electronic] Canada
• PMID: 23984825 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Apoptosis Regulatory Proteins
• Chromones
• RNA, Messenger
• Reactive Oxygen Species
• farrerol
• Malondialdehyde
• Hydrogen Peroxide
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Cell Line
• Cell Survival (drug effects)
• Chromones (pharmacology)
• Cytoprotection
• Dose-Response Relationship, Drug
• Endothelial Cells (drug effects, metabolism, pathology)
• Gene Expression Regulation
• Glutathione Peroxidase (metabolism)
• Humans
• Hydrogen Peroxide (toxicity)
• Malondialdehyde (metabolism)
• Oxidative Stress (drug effects)
• RNA, Messenger (metabolism)
• Reactive Oxygen Species (metabolism)
• Superoxide Dismutase (metabolism)