Combinational delivery of lipid-enveloped polymeric nanoparticles carrying different peptides for anti-tumor immunotherapy.

Abstract

AIM:

The authors aimed to investigate whether nanotechnology-based delivery of antigenic peptides is feasible for efficiently inducing anti-tumor cytotoxic T lymphocyte responses through vaccination.

MATERIALS & METHODS:

Three different murine melanoma antigens were entrapped in lipid-coated poly(D,L-lactide-co-glycolide) nanoparticles (NPs) by the double emulsion method.

RESULTS:

The loading efficiency of hydrophilic peptides was greatly improved when lipids were introduced to formulate lipid-coated NPs. The lipid-coated NPs carrying a single peptide and/or combinations of multiple lipid-coated NPs carrying antigenic peptides were characterized in vitro and in vivo in a C57/BL6 (B6) mouse model. Both the single melanoma antigen peptide-loaded NPs and combinational delivery of lipid-coated NPs carrying different peptides could induce antigen-specific T-cell responses. However, single peptide-loaded NPs failed to significantly delay the growth of subcutaneously inoculated B16 melanoma cells in a prophylactic setting. By contrast, the combinational delivery of lipid-coated NPs carrying different peptides significantly suppressed growth of inoculated B16 melanoma cells.

• Authors: Songwei Tan, Tetsuro Sasada, Anna Bershteyn, Kunyu Yang, Tetsuya Ioji, Zhiping Zhang
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 9 Issue 5 Pg. 635-47 (Apr 2014) ISSN: 1748-6963 [Electronic] England
• PMID: 23905577 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Lipids
• Nanocapsules
• Peptides
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid

Topics

• Animals
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage, immunology)
• Cancer Vaccines (administration & dosage, immunology)
• Cell Line, Tumor
• Lactic Acid (chemistry)
• Lipids (chemistry)
• Melanoma (immunology, pathology, prevention & control)
• Mice
• Mice, Inbred C57BL
• Nanocapsules (chemistry, ultrastructure)
• Peptides (administration & dosage, immunology)
• Polyglycolic Acid (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Survival Rate
• Treatment Outcome