Mapping the redox state of CHOP-treated non-Hodgkin's lymphoma xenografts in mice.

Abstract

Drug treatment may alter the metabolism of cancer cells and may alter the mitochondrial redox state. Using the redox scanner that collects the fluorescence signals from both the oxidized flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tumor tissues, we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice in the treatment group were treated with CHOP - cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) using the following regimen: CHO administration on day 1 followed by prednisone administration on day 1-5. On day 5 the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.049), and the Fp content of the treated group was significantly decreased (p = 0.033).

• Authors: He N Xu, Tahreem A Mir, Seung-Cheol Lee, Min Feng, Namisa Farhad, Regine Choe, Jerry D Glickson, Lin Z Li
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 789 Pg. 243-249 ( 2013) ISSN: 0065-2598 [Print] United States
• PMID: 23852501 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• NAD
• Vincristine
• Doxorubicin
• Cyclophosphamide
• Prednisone

Topics

• Animals
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage, pharmacology)
• Cell Line, Tumor
• Cyclophosphamide (administration & dosage)
• Doxorubicin (administration & dosage)
• Female
• Humans
• Lymphoma, Large B-Cell, Diffuse (drug therapy, metabolism)
• Lymphoma, Non-Hodgkin (drug therapy, metabolism)
• Mice
• Mice, Nude
• Mitochondria (drug effects, metabolism)
• NAD (metabolism)
• Oxidation-Reduction
• Prednisone (administration & dosage)
• Vincristine (administration & dosage)
• Xenograft Model Antitumor Assays