Feruloyl-L-arabinose attenuates migration, invasion and production of reactive oxygen species in H1299 lung cancer cells.

Abstract

Ferulic acid (FA), a phenolic compound, is an abundant dietary antioxidant and exerts the mitogenic effect on cells. Recently, we isolated an active FA derivative, namely feruloyl-L-arabinose (FAA), from coba husk. The aim of this study was to investigate the effects of FAA on the proliferation, migration and invasion of H1299 human lung cancer cells. Our results showed a strong antioxidant potential of FAA. Additionally, FAA inhibited the migration and invasion ability, while causing a significant accumulation of G2/M-population, of H1299 tumor cells in a dose-dependent manner, whereas no significant change on cell proliferation was observed. Results from the wound healing assay revealed that cell migration ability was markedly inhibited by FAA treatments. Similarly, results of gelatin zymography study showed that FAA treatments significantly decreased the activities of matrix metalloproteinase (MMP)-2 and MMP-9, suggesting that FAA-mediated inhibition on migration and invasion of lung cancer cells may be achieved by the down-regulation of the MMPs activities. Taken together, our present work provides a new insight into the novel inhibitory function of FAA on cell migration in H1299 cells, suggesting its promising role in the chemoprevention of lung cancer.

• Authors: Hsin-Yu Fang, Hui-Min Wang, Kuo-Feng Chang, Huei-Ting Hu, Lian-Je Hwang, Tzu-Fun Fu, Yin-Chieh Lin, Wei-Chiao Chang, Tsu-Pei Chiu, Zhi-Hong Wen, Yao Fong, Chien-Chih Chiu, Bing-Hung Chen
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 58 Pg. 459-66 (Aug 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23707472 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Coumaric Acids
• Free Radical Scavengers
• Reactive Oxygen Species
• feruloylarabinose
• Arabinose

Topics

• Antioxidants (pharmacology)
• Arabinose (analogs & derivatives, pharmacology)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Coumaric Acids (pharmacology)
• Drug Screening Assays, Antitumor
• Free Radical Scavengers (pharmacology)
• Humans
• Lung Neoplasms (metabolism, pathology)
• Neoplasm Invasiveness (prevention & control)
• Reactive Oxygen Species (metabolism)
• Spectrometry, Mass, Electrospray Ionization