Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. They participate in a wide variety of biological processes, including apoptosis, proliferation and metastasis. The aberrant expression of miRNAs has been found to play an important role in many cancers.

RESULTS:

To understand the roles of miRNAs in the bone metastasis of lung adenocarcinoma, we constructed two small RNA libraries from blood of lung adenocarcinoma patients with and without bone metastasis. High-throughput sequencing combined with differential expression analysis identified that 7 microRNAs were down-regulated and 21 microRNAs were up-regulated in lung adenocarcinoma with bone metastasis. A total of 797 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in bone metastasis, survival of the primary origin and metastatic angiogenesis of lung adenocarcinoma. These include the MAPK, Wnt, and NF-kappaB signaling pathways, as well as pathways involving the matrix metalloproteinase, cytoskeletal protein and angiogenesis factors.

CONCLUSIONS:

This study provides some insights into the molecular mechanisms that underlie lung adenocarcinoma development, thereby aiding the diagnosis and treatment of the disease.

• Authors: Lin Xie, Zuozhang Yang, Guoqi Li, Lida Shen, Xudong Xiang, Xuefeng Liu, Da Xu, Lei Xu, Yanjin Chen, Zhao Tian, Xin Chen
• Journal: PloS one (PLoS One) Vol. 8 Issue 4 Pg. e61212 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 23593434 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MicroRNAs

Topics

• Adenocarcinoma (pathology)
• Adenocarcinoma of Lung
• Bone Neoplasms (genetics, secondary)
• Female
• Genome, Human (genetics)
• Genomics
• High-Throughput Nucleotide Sequencing
• Humans
• Lung Neoplasms (pathology)
• Male
• MicroRNAs (genetics)
• Middle Aged
• Molecular Sequence Annotation
• Sequence Analysis, RNA
• Transcriptome