Abstract | BACKGROUND:
Hirsutanol A is a novel sesquiterpene compound purified from fungus Chondrostereum sp. in Sarcophyton tortuosum. Our previous studies had demonstrated that hirsutanol A exhibited potent cytotoxic effect on many kinds of cancer cell lines. In the current study, the antitumor activity of hirsutanol A and its molecular mechanisms were investigated. METHODS:
Hirsutanol A induced growth inhibition and apoptotic cell death of human colon cancer SW620 cells and human breast cancer MDA-MB-231cells were determined using MTT assay and flow cytometry assay, respectively. The effect of hirsutanol A on intrinsic ROS level and change in mitochondrial membrane potential (△ψm) of different cell lines were also measured by flow cytometry assay. The function of JNK was compromised by JNK siRNA or JNK inhibitor SP600125. The expression of cytochrome c, p-JNK, p-c-Jun after treatment with hirsutanol A were detected by Western blot analysis. Finally, the in vivo anti- tumor effect of hirsutanol A was examined in human cancer cell SW620 xenograft model. RESULTS: CONCLUSION: These data suggested that hirsutanol A inhibited tumor growth through triggering ROS production and apoptosis.
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Authors | Fen Yang, Wen-Dan Chen, Rong Deng, Hui Zhang, Jun Tang, Ke-Wei Wu, Dan-Dan Li, Gong-Kan Feng, Wen-Jian Lan, Hou-Jin Li, Xiao-Feng Zhu |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 11
Pg. 32
(Feb 08 2013)
ISSN: 1479-5876 [Electronic] England |
PMID | 23394457
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Reactive Oxygen Species
- Sesquiterpenes
- Tetrazolium Salts
- Thiazoles
- hirsutanol A
- Cytochromes c
- thiazolyl blue
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Topics |
- Agaricales
(metabolism)
- Animals
- Apoptosis
- Cell Death
- Cell Line, Tumor
- Cytochromes c
(metabolism)
- Cytosol
(metabolism)
- Flow Cytometry
- Humans
- Membrane Potentials
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Mitochondria
(metabolism)
- Neoplasm Transplantation
- Reactive Oxygen Species
(metabolism)
- Sesquiterpenes
(pharmacology)
- Signal Transduction
- Tetrazolium Salts
(pharmacology)
- Thiazoles
(pharmacology)
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