Biological assay of a novel quinoxalinone with antimalarial efficacy on Plasmodium yoelii yoelii.

Abstract

Compound 1-methyl-7-nitro-4-(5-(piperidin-1-yl)pentyl)-3,4-dihydroquinoxalin-2(1H)-one (VAM2-6) was evaluated against a blood-induced infection with chloroquine-sensitive Plasmodium yoelii yoelii lethal strain in CD1 mice in a 4-day test scheme. LD50 of the compound was 56.51 mg/kg and LD10 was 20.58 mg/kg (taken as the highest dose). Animals were treated by oral gavage of 20, 10, and 5 mg/kg. Mice in the untreated control group showed a progressively increasing parasitemia leading to mouse death on 6 days post-infection; in this group, all mice showed parasites in the blood on the fifth day of sampling; the mean parasitemia on that day was 19.4%. A 4-day dosage of 20 mg/kg of VAM2-6 showed a 97% chemosuppression of total parasitemia on the fifth day, a 28 days survival time, and 20% of cured animals. A 4-day dosage of 10 and 5 mg/kg showed 85 and 37%, respectively, chemosuppression of total parasitemia on the fifth day; but all mice died from days 6 to 9 post-infection with increasing parasitemia. Mice treated with chloroquine at 5 mg/kg survived during the experiment. The results obtained in this study showed that the infection outcome of P. yoelii yoelii-infected mice is affected by VAM2-6 compound by slowing down the parasite replication, retarding the patency time, and increasing their survival time. Although compound VAM2-6 was active at higher doses than chloroquine, these results leaves a door open to the study of its structure in order to improve its antimalarial activity.

• Authors: Norma Rivera, Yovani Marrero Ponce, Vicente J Arán, Cecilia Martínez, Filiberto Malagón
• Journal: Parasitology research (Parasitol Res) Vol. 112 Issue 4 Pg. 1523-7 (Apr 2013) ISSN: 1432-1955 [Electronic] Germany
• PMID: 23338979 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimalarials
• Quinoxalines

Topics

• Administration, Oral
• Animals
• Antimalarials (therapeutic use)
• Biological Assay
• Disease Models, Animal
• Malaria (drug therapy)
• Male
• Mice
• Plasmodium yoelii (drug effects)
• Quinoxalines (therapeutic use)
• Survival Analysis
• Treatment Outcome