Adherens junctions (AD and tight junctions (TJ) play a key role in maintaining the apical-basolateral polarity and cohesive structure of epithelial cells. These epithelial junctions are maintained by the interaction of several key
proteins including
E-cadherin,
claudins,
occludin and
zona-occludens. The zinc finger
protein, Snail, has previously been identified as a possible regulator of several of these AJ and TJ
proteins. Expression levels of ZO-1,
occludin,
claudins,
E-cadherin, human Scribble
protein and Snail were determined in HeLa and CaSki cervical
carcinoma cell lines by imuunohistochemistry (IHC) and Western blotting. In tandem, tissue microarrays were utilised in the IHC-based detection of
E-cadherin in 26 cases of non-cancerous cervical tissue, 15 cases of
cervical intraepithelial neoplasia 1 (CIN1), 11 cases of CIN2, 12 cases of CIN3, 50 cases of
squamous cell carcinoma and two cases of
adenocarcinoma. This study found aberrant
E-cadherin expression in CIN lesions and cases of
squamous cell carcinoma compared to normal epithelium. HeLa cells were
E-cadherin-negative while CaSki cells were positive, with HeLa cells showing a high level of Snail expression.
Occludin and ZO-1 expression was detected in both cell lines. No expression was observed for
claudin 1 or
claudin 5 tight junction proteins in HeLa cells or CaSki cells. Loss of expression of
claudin 1,
claudin 5 and
E-cadherin, with concomitant increase in Snail may be associated with loss of epithelial cell polarity and alterations in intercellular adhesion.