Enhancement of the immunogenicity and protective efficacy of a mucosal influenza subunit vaccine by the saponin adjuvant GPI-0100.

Abstract

Identification of safe and effective adjuvants remains an urgent need for the development of inactivated influenza vaccines for mucosal administration. Here, we used a murine challenge model to evaluate the adjuvant activity of GPI-0100, a saponin-derived adjuvant, on influenza subunit vaccine administered via the intranasal or the intrapulmonary route. Balb/c mice were immunized with 1 µg A/PR/8 (H1N1) subunit antigen alone or in combination with varying doses of GPI-0100. The addition of GPI-0100 was required for induction of mucosal and systemic antibody responses to intranasally administered influenza vaccine and significantly enhanced the immunogenicity of vaccine administered via the intrapulmonary route. Remarkably, GPI-0100-adjuvanted influenza vaccine given at a low dose of 2×1 µg either in the nares or directly into the lungs provided complete protection against homologous influenza virus infection.

• Authors: Heng Liu, Harshad P Patil, Jacqueline de Vries-Idema, Jan Wilschut, Anke Huckriede
• Journal: PloS one (PLoS One) Vol. 7 Issue 12 Pg. e52135 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 23284901 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• GPI0100
• Immunoglobulin A, Secretory
• Immunoglobulin G
• Influenza Vaccines
• Saponins
• Vaccines, Subunit

Topics

• Adjuvants, Immunologic
• Administration, Intranasal
• Animals
• Antibodies, Viral (blood, immunology)
• Female
• Immunoglobulin A, Secretory (immunology)
• Immunoglobulin G (blood, immunology)
• Influenza A Virus, H1N1 Subtype (immunology)
• Influenza Vaccines (administration & dosage, immunology)
• Lung (immunology, pathology, virology)
• Mice
• Mucous Membrane (immunology)
• Orthomyxoviridae Infections (prevention & control, virology)
• Saponins (immunology)
• T-Lymphocytes, Helper-Inducer (immunology)
• Vaccines, Subunit