[Diagnostic value of cytokeratin 19 fragment in nasopharyngeal carcinoma].

Abstract

OBJECTIVE:

To explore the clinical significance of cytokeratin 19 fragments test in the diagnosis of nasopharyngeal carcinoma.

METHODS:

The study included 102 cases of nasopharyngeal carcinoma, 90 cases of nasal polyp/nasopharyngitis, and 150 healthy individuals. RT-PCR was used to detect CK19 mRNA expression and Western blot to detect CK19 fragment protein expression in tissues of nasopharyngeal carcinoma. Expression of CK19-2G2 was examined by immunohistochemistry. Chemiluminescence analysis was used to detect the serum levels of CK19-2G2, and ELISA to detect that of EB-VCA IgA.

RESULTS:

Among 102 cases of nasophryngeal carcinoma, 64 showed CK19 mRNA expression by RT-PCR, 60 showed CK19 protein fragments in tumor tissues by Western blot, and 66 showed expression of CK19-2G2 by immunohistochemistry in nasopharyngeal carcinoma, including strong positivity in 20 cases, moderate in 34 cases and weak in 12 cases. The sensitivity and specificity of CK19-2G2 in the diagnosis of nasopharyngeal carcinoma were 49.0% and 89.2%, and those of EB-VCA IgA were 52.9% and 85.4%, respectively. The combined detection of CK19-2G2 and EB-VCA IgA increased the sensitivity to 73.5% while the specificity remained at 80.0%.

CONCLUSIONS:

High levels of CK19-2G2 fragment expressed in tissue and serum are present in patients with nasopharyngeal carcinoma. The serum level of CK19-2G2 is helpful in the diagnosis of nasopharyngeal carcinoma. Furthermore, the combination of serum CK19-2G2 and EB-VCA IgA improves the detection sensitivity.

• Authors: Dan-sheng Lei, Jing Yu, Xian-li Tong, Ming-wei Wang, Kun Wang, Hu Chen
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 41 Issue 7 Pg. 461-5 (Jul 2012) ISSN: 0529-5807 [Print] China
• PMID: 22932457 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigens, Viral
• Capsid Proteins
• Epstein-Barr viral capsid antigen
• Immunoglobulin A
• Keratin-19
• Peptide Fragments
• RNA, Messenger

Topics

• Adult
• Aged
• Antigens, Viral (blood)
• Blotting, Western
• Capsid Proteins (blood)
• Carcinoma, Squamous Cell (diagnosis, metabolism, pathology)
• Herpesvirus 4, Human (immunology)
• Humans
• Immunoglobulin A (blood)
• Immunohistochemistry
• Keratin-19 (blood, metabolism)
• Middle Aged
• Nasopharyngeal Neoplasms (diagnosis, metabolism, pathology)
• Neoplasm Staging
• Peptide Fragments (blood, metabolism)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Sensitivity and Specificity