Abstract | OBJECTIVE: METHODS AND RESULTS: In primary cultured AFs, VEGF increased intracellular and secreted OPN expression in a time- and dose-dependent manner, which was effectively suppressed by a specific anti-Flt-1 hexapeptide. Interestingly, VEGF treatment of AFs enhanced the capability of AF- conditioned medium to stimulate macrophages chemotaxis, and this effect was attenuated after blockade of OPN from AF- conditioned medium. Furthermore, perivascular delivery of anti-Flt-1 peptide preferentially concentrated in the adventitia resulted in a decrease of neointima formation after balloon injury in carotid arteries. The inhibition of neointima formation was preceded by significant reduction of VEGF and OPN expression with concurrent macrophage infiltration into adventitia after injury. Activation of extracellular signal-regulated kinase 1/2 pathway was involved in OPN upregulation and macrophage chemotaxis. CONCLUSIONS:
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Authors | Xiao-Dong Li, Jing Chen, Cheng-Chao Ruan, Ding-Liang Zhu, Ping-Jin Gao |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 32
Issue 9
Pg. 2250-8
(Sep 2012)
ISSN: 1524-4636 [Electronic] United States |
PMID | 22814749
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Oligopeptides
- Protein Kinase Inhibitors
- Spp1 protein, rat
- Vascular Endothelial Growth Factor A
- vascular endothelial growth factor A, rat
- Osteopontin
- Flt1 protein, rat
- Vascular Endothelial Growth Factor Receptor-1
- Mapk1 protein, rat
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
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Topics |
- Animals
- Aorta, Thoracic
(immunology, metabolism, pathology)
- Carotid Artery Injuries
(immunology, metabolism, pathology)
- Carotid Artery, Common
(immunology, metabolism, pathology)
- Cell Line
- Cell Proliferation
- Chemotaxis
- Connective Tissue
(drug effects, immunology, metabolism, pathology)
- Disease Models, Animal
- Fibroblasts
(immunology, metabolism, pathology)
- Inflammation
(immunology, metabolism, pathology)
- Macrophages
(immunology, metabolism)
- Male
- Mice
- Mitogen-Activated Protein Kinase 1
(antagonists & inhibitors, metabolism)
- Mitogen-Activated Protein Kinase 3
(antagonists & inhibitors, metabolism)
- Neointima
- Oligopeptides
(pharmacology)
- Osteopontin
(metabolism)
- Protein Kinase Inhibitors
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
- Time Factors
- Up-Regulation
- Vascular Endothelial Growth Factor A
(metabolism)
- Vascular Endothelial Growth Factor Receptor-1
(antagonists & inhibitors, metabolism)
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