Effect of ergosta-4,6,8(14),22-tetraen-3-one (ergone) on adenine-induced chronic renal failure rat: a serum metabonomic study based on ultra performance liquid chromatography/high-sensitivity mass spectrometry coupled with MassLynx i-FIT algorithm.

Abstract

BACKGROUND:

Ergosta-4,6,8(14),22-tetraen-3-one (ergone) has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. We investigated the therapeutic effects and mechanism of ergone on a chronic renal failure model of rats induced by adenine.

METHODS:

A serum metabonomic method based on the UPLC Q-TOF/MS was undertaken to explore the excretion pattern of low molecular mass metabolites.

RESULTS:

Coupled with blood biochemistry and kidney histopathology results, the significant difference in metabolic profiling between adenine-induced chronic renal failure group and the ergone treated group by using pattern recognition analysis indicated that changes in global serum metabolites occurred. Some significantly changed metabolites like lysophosphatidylcholines, adenine, dopamine, creatinine, aspartic acid and phenylalanine have been found and identified. These biochemical changes in serum metabolites are related to the perturbations of amino acid metabolism and lecithin metabolism, which may be helpful to further understand the chronic renal failure and therapeutic mechanisms of ergone.

CONCLUSION:

The work shows that the metabonomic method is a valuable tool for studying the essence of chronic kidney disease and therapeutic effect mechanism of preclinical or clinical drug.

• Authors: Ying-Yong Zhao, Xian-Long Cheng, Ji-Hong Cui, Xing-Rong Yan, Feng Wei, Xu Bai, Rui-Chao Lin
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 413 Issue 19-20 Pg. 1438-45 (Oct 09 2012) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 22687646 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Cholestenones
• Lysophosphatidylcholines
• Aspartic Acid
• Phenylalanine
• Creatinine
• Adenine
• ergosta-4,6,8(14),22-tetraen-3-one
• Dopamine

Topics

• Adenine (adverse effects, blood)
• Animals
• Aspartic Acid (blood)
• Cholestenones (administration & dosage)
• Chromatography, High Pressure Liquid
• Creatinine (blood)
• Dopamine (blood)
• Kidney (drug effects, metabolism, pathology)
• Kidney Failure, Chronic (chemically induced, metabolism, prevention & control)
• Lipid Metabolism
• Lysophosphatidylcholines (blood)
• Male
• Metabolomics
• Phenylalanine (blood)
• Principal Component Analysis
• Rats
• Rats, Sprague-Dawley
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization