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A sensitive fluorescence strategy for telomerase detection in cancer cells based on T7 exonuclease-assisted target recycling amplification.

Abstract
A sensitive fluorescence strategy based on T7 exonuclease-assisted target recycling amplification was developed for telomerase detection in cancer cells. The novel strategy improved the fluorescence signal and sensitivity compared with the previously reported methods.
AuthorsHai-bo Wang, Shuang Wu, Xia Chu, Ru-Qin Yu
JournalChemical communications (Cambridge, England) (Chem Commun (Camb)) Vol. 48 Issue 47 Pg. 5916-8 (Jun 14 2012) ISSN: 1364-548X [Electronic] England
PMID22558640 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Telomerase
  • Exodeoxyribonucleases
  • gene b exonuclease
Topics
  • Biosensing Techniques
  • Cell Line, Tumor
  • Exodeoxyribonucleases (metabolism)
  • Humans
  • Neoplasms (enzymology)
  • Spectrometry, Fluorescence
  • Telomerase (metabolism)

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