Abstract | AIM: METHODS: In vitro, the HL-60 cells were cultured and exposed to NM at doses 0-1000 μg/ml. Cell viability was assessed by Trypan blue dye exclusion test, matrix metalloproteinases ( MMP) expression by gelatinase zymography, invasion through Matrigel and apoptosis by live green Poly Caspase Detection Kit. In vivo studies were carried out in athymic nude mice subcutaneously inoculated with HL-60 cells. RESULTS: In vitro, NM exhibited a dose dependent reduction in cells viability. Zymography revealed matrix MMP-2 and phorbol 12-myristate 13-acetate induced MMP-9 expression. NM inhibited expression of both MMP in a dose dependent manner. Similar step-wise reduction in the Matrigel invasion by HL-60 cells was also observed by this combination with incremental doses. Gradually increasing doses of NM induced significant apoptosis in HL-60 cells. In vivo, NM inhibited tumor growth by 50%. CONCLUSION: The results indicate that NM significantly suppresses tumor growth, decreases cell viability, inhibits MMP expression, Matrigel invasion and induces apoptosis in HL-60 cells.
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Authors | M W Roomi, N W Roomi, B Bhanap, M Rath, A Niedzwiecki |
Journal | Experimental oncology
(Exp Oncol)
Vol. 33
Issue 4
Pg. 212-5
(Dec 2011)
ISSN: 2312-8852 [Electronic] Ukraine |
PMID | 22217709
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Complex Mixtures
- Gelatinases
- Matrix Metalloproteinases
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage)
- Apoptosis
(drug effects)
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Complex Mixtures
(administration & dosage, pharmacology)
- Dietary Supplements
- Gelatinases
(metabolism)
- HL-60 Cells
- Humans
- Leukemia, Promyelocytic, Acute
(drug therapy, enzymology)
- Male
- Matrix Metalloproteinases
(metabolism)
- Mice
- Mice, Nude
- Nutritional Physiological Phenomena
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