Identification and characterization of novel immunomodulatory bursal-derived pentapeptide-II (BPP-II).

Abstract

The bursa of Fabricius, the acknowledged central humoral immune organ, plays a vital role in B lymphocyte differentiation. However, there are few reports of the molecular basis of the mechanism on immune induction and potential antitumor activity of bursal-derived peptides. In this paper, a novel bursal-derived pentapeptide-II (BPP-II, MTLTG) was isolated and exerted immunomodulatory functions on antibody responses in vitro. Gene microarray analyses demonstrated that BPP-II regulated expression of 2478 genes in a mouse-derived hybridoma cell line. Immune-related gene ontology functional procedures were employed for further functional analysis. Furthermore, the majority of BPP-II-regulated pathways were associated with immune responses and tumor processes. Moreover, BPP-II exhibited immunomodulatory effects on antigen-specific immune responses in vivo, including enhancement of avian influenza virus (H9N2 subtype)-specific antibody and cytokine production and modification of T cell immunophenotypes and lymphocyte proliferation. Finally, BPP-II triggered p53 expression and stabilization and selectively inhibited tumor cell proliferation. These data identified the multifunctional factor, BPP-II, as a novel biomaterial representing an important linking between the humoral central immune system and immune induction, including antitumor. Information generated in this study elucidates further the mechanisms involved in humoral immune system and represents the potential basis of effective immunotherapeutic strategies for treating human tumors and immune improvement.

• Authors: Xiu-Li Feng, Qing-Tao Liu, Rui-Bing Cao, Bin Zhou, Zhi-Yong Ma, Wen-Lei Deng, Jian-Chao Wei, Ya-Feng Qiu, Fang-Quan Wang, Jin-Yan Gu, Feng-Juan Wang, Qi-Sheng Zheng, Hassan Ishag, Pu-Yan Chen
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 287 Issue 6 Pg. 3798-807 (Feb 03 2012) ISSN: 1083-351X [Electronic] United States
• PMID: 22184121 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Cytokines
• Immunologic Factors
• Oligopeptides
• TP53 protein, human
• Tumor Suppressor Protein p53

Topics

• Animals
• Antibodies, Viral (immunology)
• Bursa of Fabricius (chemistry, immunology)
• Cell Line, Tumor
• Chickens (immunology)
• Cytokines (immunology)
• Female
• Humans
• Immunologic Factors (chemistry, immunology, isolation & purification, pharmacology)
• Influenza A Virus, H9N2 Subtype (metabolism)
• Mice
• Mice, Inbred BALB C
• Neoplasms (drug therapy, immunology, pathology)
• Oligopeptides (chemistry, immunology, isolation & purification, pharmacology)
• T-Lymphocytes (immunology)
• Tumor Suppressor Protein p53 (immunology)