Abstract | OBJECTIVE: METHODS: RESULTS: Increased IgG reactivity with activated neutrophils, particularly binding to NETs, was paralleled by preferential binding to deiminated histones over nondeiminated histones by ELISA in a majority of sera from FS patients but only in a minority of sera from SLE and RA patients. Immunoblotting revealed autoantibody preference for deiminated histones H3, H4, and H2A in most FS patients and in a subset of SLE and RA patients. In patients with AAVs, serum IgG preferentially bound nondeiminated histones over deiminated histones. Increased levels of deiminated histones were detected in neutrophils from RA patients. CONCLUSION: Circulating autoantibodies in FS are preferentially directed against PAD-4-deiminated histones and bind to activated neutrophils and NETs. Thus, increased reactivity with modified autoantigens in FS implies a direct contribution of neutrophil activation and the production of NET-associated nuclear autoantigens in the initiation or progression of FS.
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Authors | Nishant Dwivedi, Jagriti Upadhyay, Indira Neeli, Salar Khan, Debendra Pattanaik, Linda Myers, Kyriakos A Kirou, Bernhard Hellmich, Bryan Knuckley, Paul R Thompson, Mary K Crow, Ted R Mikuls, Elena Csernok, Marko Radic |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 64
Issue 4
Pg. 982-92
(Apr 2012)
ISSN: 1529-0131 [Electronic] United States |
PMID | 22034172
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 by the American College of Rheumatology. |
Chemical References |
- Autoantibodies
- Autoantigens
- Histones
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
(immunology)
- Arthritis, Rheumatoid
(immunology)
- Autoantibodies
(immunology)
- Autoantigens
(immunology)
- Felty Syndrome
(immunology)
- Female
- Histones
(immunology)
- Humans
- Lupus Erythematosus, Systemic
(immunology)
- Male
- Middle Aged
- Neutrophils
(immunology)
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