Evidence for the involvement of vicinal sulfhydryl groups in the insulin stimulation of intracellular glucose metabolism in Zajdela hepatoma cells.

Abstract

Phenylarsine oxide (PhAsO), a dithiol reagent that blocks insulin stimulation of glucose transport in 3T3 L1 cells, also altered insulin stimulation of intracellular glucose metabolism in Zajdela Hepatoma cultured cells. PhAsO (2 microM) similarly inhibited the insulin-induced glycogen and lipid syntheses without modifying the basal level of these processes, cell viability or the ATP content. Prior incubation of the cells with PhAsO did not prevent insulin binding to the cells, or activation of the receptor tyrosine kinase, while it minimally (16%) altered receptor internalization. These results indicate that cellular dithiols located at a post-receptor step are involved in the transduction of the insulin signal to intracellular glucose metabolism.

• Authors: M Caron, G Cherqui, D Wicek, B Melin, C Reynet, J Capeau, J Picard
• Journal: Bioscience reports (Biosci Rep) Vol. 10 Issue 1 Pg. 23-9 (Feb 1990) ISSN: 0144-8463 [Print] England
• PMID: 2187543 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Arsenicals
• Insulin
• Insulin Antagonists
• Lipids
• Liver Glycogen
• Sulfhydryl Compounds
• Sulfhydryl Reagents
• oxophenylarsine
• Adenosine Triphosphate
• L-Lactate Dehydrogenase
• Protein-Tyrosine Kinases
• Receptor, Insulin
• Glucose

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Arsenicals (pharmacology)
• Glucose (metabolism)
• Insulin (pharmacology)
• Insulin Antagonists
• L-Lactate Dehydrogenase (metabolism)
• Lipids (biosynthesis)
• Liver Glycogen (biosynthesis)
• Liver Neoplasms, Experimental (metabolism)
• Protein-Tyrosine Kinases (metabolism)
• Receptor, Insulin (drug effects)
• Sulfhydryl Compounds (metabolism)
• Sulfhydryl Reagents (pharmacology)
• Tumor Cells, Cultured