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Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome.

Abstract
The VlsE lipoprotein of Borrelia burgdorferi elicits a strong immune response during the course of Lyme disease. The present study was aimed at characterization of the epitopes of VlsE targeted by the antibody response in patients with post-Lyme disease syndrome, a condition characterized by persisting symptoms of pain, fatigue, and/or neurocognitive impairment despite antibiotic treatment of B. burgdorferi infection. Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. The crystal structure of VlsE indicated that the newly described epitopes form a contiguous region in the surface-exposed membrane-proximal part of the monomeric form of the protein.
AuthorsAbhishek Chandra, Norman Latov, Gary P Wormser, Adriana R Marques, Armin Alaedini
JournalClinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 141 Issue 1 Pg. 103-10 (Oct 2011) ISSN: 1521-7035 [Electronic] United States
PMID21778118 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural)
CopyrightCopyright © 2011 Elsevier Inc. All rights reserved.
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Epitopes, B-Lymphocyte
  • Lipoproteins
  • VlsE protein, Borrelia burgdorferi
Topics
  • Adult
  • Aged
  • Amino Acid Sequence
  • Antibodies, Bacterial (blood)
  • Antigens, Bacterial (chemistry, genetics, immunology)
  • Bacterial Proteins (chemistry, genetics, immunology)
  • Borrelia burgdorferi (genetics, immunology)
  • Epitope Mapping
  • Epitopes, B-Lymphocyte (chemistry, genetics, immunology)
  • Female
  • Humans
  • Lipoproteins (chemistry, genetics, immunology)
  • Lyme Neuroborreliosis (immunology, microbiology)
  • Male
  • Middle Aged
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Array Analysis
  • Syndrome

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