Abstract | BACKGROUND:
Bladder cancer is a significant healthcare problem in the United States of America with a high recurrence rate. Early detection of bladder cancer is essential for removing the tumor with preservation of the bladder, avoiding metastasis and hence improving prognosis and long-term survival. The objective of this study was to analyze the presence of DEK protein in voided urine of bladder cancer patients as a urine-based bladder cancer diagnostic test. METHODS: RESULTS: The DEK protein is expressed in 33 of 38 bladder tumor tissues with no expression in adjacent normal tissue. Based on our sample size, DEK protein is expressed in 100% of tumors of low malignant potential, 92% of tumors of low grade and in 71% of tumors of high grade. Next, we analyzed 42 urine samples from patients with active TCC, other malignant urogenital disease, non-malignant urogenital disease and healthy individuals for DEK protein expression by western blot analysis. We are the first to show that the DEK protein is present in the urine of bladder cancer patients. Approximately 84% of TCC patient urine specimens were positive for urine DEK. CONCLUSION:
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Authors | Antara Datta, Martin E Adelson, Yakov Mogilevkin, Eli Mordechai, Abraham A Sidi, Jason P Trama |
Journal | BMC cancer
(BMC Cancer)
Vol. 11
Pg. 234
(Jun 10 2011)
ISSN: 1471-2407 [Electronic] England |
PMID | 21663673
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Chromosomal Proteins, Non-Histone
- Dek protein, human
- Oncogene Proteins
- Poly-ADP-Ribose Binding Proteins
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Topics |
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(metabolism, urine)
- Cell Line, Tumor
- Chromosomal Proteins, Non-Histone
(metabolism, urine)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Male
- Middle Aged
- Oncogene Proteins
(metabolism, urine)
- Poly-ADP-Ribose Binding Proteins
- Urinary Bladder Neoplasms
(diagnosis)
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