Effect of NADPH-oxidase inhibitors in the experimental model of zymosan-induced shock in mice.

Abstract

The aim of this study was to investigate the effects of NADPH-oxidase inhibitors, in a mouse model of zymosan. Zymosan-induced shock was induced in mice by administration of zymosan (500 mg/kg, i.p.). The pharmacological treatment was the administration of apocynin (5 mg/kg 10% DMSO i.p.) and diphenylene iodonium chloride (DPI) (1 mg/kg i.v.) 1 h and 6 h after zymosan administration. MOF and systemic inflammation in mice was assessed 18 h after administration of zymosan. NADPH-oxidase inhibitors caused a significant reduction of the (1) peritoneal exudate formation, (2) neutrophil infiltration, (3) multiple organ dysfunction syndrome, (4) nitrotyrosine, (5) poly (ADP-ribose) (PAR), (6) cytokine formation, (7) adhesion molecule expression, (8) nuclear factor (NF-κB) expression and (9) apoptosis induced by zymosan. Moreover, NADPH-oxidase inhibitors treatment significantly reduced the systemic toxicity, the loss in body weight and the mortality caused by zymosan. This study has shown that NADPH-oxidase inhibitors attenuate the degree of zymosan-induced non-septic shock in mice.

• Authors: Daniela Impellizzeri, Emanuela Mazzon, Rosanna Di Paola, Irene Paterniti, Placido Bramanti, Salvatore Cuzzocrea
• Journal: Free radical research (Free Radic Res) Vol. 45 Issue 7 Pg. 820-34 (Jul 2011) ISSN: 1029-2470 [Electronic] England
• PMID: 21623687 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Informa UK, Ltd.

Chemical References

• Acetophenones
• Cytokines
• Enzyme Inhibitors
• Nitrates
• Nitrites
• Onium Compounds
• Reactive Oxygen Species
• diphenyleneiodonium
• Zymosan
• acetovanillone
• NADPH Oxidases

Topics

• Acetophenones (pharmacology)
• Animals
• Apoptosis (drug effects)
• Cytokines (biosynthesis)
• Enzyme Inhibitors (therapeutic use)
• Male
• Mice
• Multiple Organ Failure (chemically induced, drug therapy)
• NADPH Oxidases (antagonists & inhibitors)
• Nitrates (blood)
• Nitrites (blood)
• Onium Compounds (pharmacology)
• Reactive Oxygen Species
• Shock (chemically induced, drug therapy)
• Systemic Inflammatory Response Syndrome (chemically induced, drug therapy)
• Zymosan