Preparation and efficacy of an inactivated subunit vaccine (NFUIBHK) against type 2 Herpes simplex virus infection.

Abstract

A vaccine against Herpes simplex virus infection was prepared by Nonidet NP 40 and formalin treatment of a type 1, infected-cell extract; virus particles were removed by ultracentrifugation over sucrose. These procedures were not detrimental to the antigenic quality of the vaccine preparation. The vaccine afforded significant protection to experimental type 2 genital herpes virus infection in mice, as adjudged by clinical observations, cytopathological change, and virus yields.

• Authors: G R Skinner, D R Williams, A Buchan, J Whitney, M Harding, K Bodfish
• Journal: Medical microbiology and immunology (Med Microbiol Immunol) Vol. 166 Issue 1-4 Pg. 119-32 (Nov 17 1978) ISSN: 0300-8584 [Print] Germany
• PMID: 214676 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Viral
• Surface-Active Agents
• Viral Vaccines
• Formaldehyde

Topics

• Animals
• Antigens, Viral (isolation & purification)
• Cell Line
• Female
• Formaldehyde (pharmacology)
• Genital Diseases, Female (prevention & control)
• Herpes Simplex (prevention & control)
• Humans
• Mice
• Simplexvirus (drug effects, immunology)
• Surface-Active Agents (pharmacology)
• Ultracentrifugation
• Viral Vaccines (immunology, isolation & purification)