p53 is important for the anti-invasion of ganoderic acid T in human carcinoma cells.

Abstract

The function of p53 induced by ganoderic acids (GAs) in anti-invasion was unknown, although our previous work reported the inhibition of tumor invasion and metastasis by Ganoderic acid T (GA-T). This work indicated that GA-T promoted cell aggregation, inhibited cell adhesion and surpressed cell migration with a dose-dependent manner in human colon tumor cell lines of HCT-116 p53(+/+) and p53(-/-). Furthermore, comparing the ratios of HCT-116 p53(+/+) and p53(-/-) cells, p53 modified GA-T inhibition of migration and adhesion and GA-T promotion of cell aggregation, and p53 also modified GA-T inhibition of NF-κB nuclear translocation, IκBα degradation, and down-regulation of urokinase-type plaminogen activator (uPA), matrix metalloproteinase-2/9 (MMP-2/9), inducible nitric oxide synthase (iNOS/NOS2) protein expression and inducible nitric oxide (NO) production. The results indicated that p53 played an important role in anti-invasion of GA-T in human carcinoma cells. p53 may be an important target for GA-T inhibiting human carcinoma cells anti-invasion.

• Authors: Nian-Hong Chen, Jian-Jiang Zhong
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 18 Issue 8-9 Pg. 719-25 (Jun 15 2011) ISSN: 1618-095X [Electronic] Germany
• PMID: 21353507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Drugs, Chinese Herbal
• I-kappa B Proteins
• NFKBIA protein, human
• TP53 protein, human
• Transcription Factor RelA
• Tumor Suppressor Protein p53
• ganoderic acid T
• NF-KappaB Inhibitor alpha
• Lanosterol
• Nitric Oxide
• NOS2 protein, human
• Nitric Oxide Synthase Type II
• Urokinase-Type Plasminogen Activator
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Adhesion (drug effects)
• Cell Aggregation (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Colonic Neoplasms (drug therapy, metabolism, pathology)
• Drugs, Chinese Herbal (pharmacology)
• HCT116 Cells
• Humans
• I-kappa B Proteins (metabolism)
• Lanosterol (analogs & derivatives, pharmacology)
• Matrix Metalloproteinase 2 (biosynthesis)
• Matrix Metalloproteinase 9 (metabolism)
• NF-KappaB Inhibitor alpha
• Neoplasm Invasiveness
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (biosynthesis)
• Phytotherapy
• Reishi (chemistry)
• Transcription Factor RelA (metabolism)
• Tumor Suppressor Protein p53 (metabolism)
• Urokinase-Type Plasminogen Activator (biosynthesis)