Immunohistochemical analysis of p53, APE1, hMSH2 and ERCC1 proteins in actinic cheilitis and lip squamous cell carcinoma.

Abstract

AIMS:

This study has compared the tissue expression of the p53 tumour suppressor protein and DNA repair proteins APE1, hMSH2 and ERCC1 in normal, dysplastic and malignant lip epithelium.

METHODS AND RESULTS:

Morphological analysis and immunohistochemistry were performed on archived specimens of normal lip mucosa (n=15), actinic cheilitis (AC) (n=30), and lip squamous cell carcinoma (LSCC) (n=27). AC samples were classified morphologically according to the severity of epithelial dysplasia and risk of malignant transformation. LSCC samples were morphologically staged according to WHO and invasive front grading (IFG) criteria. Differences between groups and morphological stages were determined by bivariate statistical analysis. Progressive increases in the percentage of epithelial cells expressing p53 and APE1 were associated with increases in morphological malignancy from normal lip mucosa to LSCC. There was also a significant reduction in epithelial cells expressing hMSH2 and ERCC1 proteins in the AC and LSCC groups. A higher percentage of malignant cells expressing APE1 was found in samples with an aggressive morphological IFG grade.

CONCLUSIONS:

Our data showed that epithelial cells from premalignant to malignant lip disease exhibited changes in the expression of p53, APE1, hMSH2 and ERCC1 proteins; these molecular change might contribute to lip carcinogenesis.

• Authors: Ludmilla R Souza, Thiago Fonseca-Silva, Camila S Pereira, Erivelton P Santos, Lucianne C Lima, Heloísa A Carvalho, Ricardo S Gomez, André L S Guimarães, Alfredo M B De Paula
• Journal: Histopathology (Histopathology) Vol. 58 Issue 3 Pg. 352-60 (Feb 2011) ISSN: 1365-2559 [Electronic] England
• PMID: 21323960 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Blackwell Publishing Limited.

Chemical References

• DNA-Binding Proteins
• Tumor Suppressor Protein p53
• ERCC1 protein, human
• Endonucleases
• MSH2 protein, human
• MutS Homolog 2 Protein
• APEX1 protein, human
• DNA-(Apurinic or Apyrimidinic Site) Lyase

Topics

• Carcinoma, Squamous Cell (metabolism, pathology)
• Cell Differentiation
• Cheilitis (metabolism, pathology)
• DNA-(Apurinic or Apyrimidinic Site) Lyase (metabolism)
• DNA-Binding Proteins (metabolism)
• Endonucleases (metabolism)
• Female
• Humans
• Immunohistochemistry
• Lip Neoplasms (metabolism, pathology)
• Male
• Middle Aged
• MutS Homolog 2 Protein (metabolism)
• Retrospective Studies
• Tumor Suppressor Protein p53 (metabolism)