Cytotoxic and antimicrobial activities of aporphine alkaloids isolated from Stephania venosa (Blume) Spreng.

Abstract

The cytotoxic activity of five alkaloids, namely 4,5-dioxo-dehydrocrebanine (1), dehydrocrebanine (2), crebanine (3), oxostephanine (4), and thailandine (5) isolated from the tuber and leaves of Stephania venosa (Blume) Spreng was investigated. Thailandine showed the strongest activity against lung carcinoma cells (A549) (IC50 of 0.30 µg/mL) with very low cytotoxicity against normal embryonic lung cells (MRC-5). Thailandine also demonstrated strong activity against Plasmodium falciparum, K1 strain (IC50 of 20 ng/mL), and Mycobacterium tuberculosis H(37)Ra (MIC of 6.25 µg/mL) as well as gram-positive bacteria such as Streptococcus pneumoniae and Staphylococcus aureus. Oxostephanine exhibited strong activity against breast cancer (BC) and acute lymphoblastic leukemia cells (MOLT-3) with an IC50 of 0.24 and 0.71 µg/mL, respectively, and exhibited very low cytotoxicity against MRC-5 cells. Dehydrocrebanine demonstrated strong activity against promyelocytic leukemia cells (HL-60) with an IC50 of 2.14 µg/mL whereas crebanine showed weak activity against cancer cell lines. However, both of them showed cytotoxicity against MRC-5 cells.

• Authors: Arthit Makarasen, Wandee Sirithana, Samang Mogkhuntod, Nisachon Khunnawutmanotham, Nitirat Chimnoi, Supanna Techasakul
• Journal: Planta medica (Planta Med) Vol. 77 Issue 13 Pg. 1519-24 (Sep 2011) ISSN: 1439-0221 [Electronic] Germany
• PMID: 21305448 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Alkaloids
• Anti-Infective Agents
• Aporphines
• Plant Extracts

Topics

• Alkaloids (chemistry, isolation & purification, pharmacology)
• Anti-Infective Agents (chemistry, isolation & purification, pharmacology)
• Aporphines (chemistry, isolation & purification, pharmacology)
• Aspergillus fumigatus (drug effects)
• Candida (drug effects)
• Cell Line, Tumor
• Female
• Gram-Positive Bacteria (drug effects)
• Herpesvirus 1, Human (drug effects)
• Humans
• Inhibitory Concentration 50
• Microbial Sensitivity Tests
• Mycobacterium tuberculosis (drug effects)
• Phialophora (drug effects)
• Plant Extracts (chemistry, pharmacology)
• Plant Leaves (chemistry)
• Plant Tubers (chemistry)
• Plasmodium falciparum (drug effects)
• Stephania (chemistry)
• Thailand