Abstract |
Our previous study demonstrated that overexpression of Id-1 (inhibitor of differentiation/ DNA binding) was associated with lymphatic metastasis in human oral squamous cell carcinoma (OSCC). In this study, we further unveiled the association of Id-1 with vascular endothelial growth factor-C ( VEGF-C) and peritumoral lymphatic vessel density (PLVD), and the effect of silencing Id-1 on inhibiting lymphangiogenesis in OSCC. We found that Id-1 was associated with VEGF-C (r=0.569, p<0.001) and PLVD (r=0.240, p<0.001) in OSCC. Lentivirus-mediated RNA interference targeting Id-1 in an OSCC cell line Tca8113 resulted in down-regulation of VEGF-C (p=0.003, 0.007). Moreover, when Id-1 was suppressed by injecting Id-1-siRNA-lentivirus into the transplanted tumors in nude mice, VEGF-C was down-regulated (p=0.018) and the PLVD decreased (p=0.001). Our results suggest that Id-1 was correlated with lymphangiogenesis in OSCC. Silencing Id-1 could inhibit lymphangiogenesis through down-regulation of VEGF-C and it might be a promising treatment modality for the lymphatic metastasis of OSCC.
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Authors | Zuoqing Dong, Fengcai Wei, Chengjun Zhou, Tomoki Sumida, Hiroyuki Hamakawa, Yingwei Hu, Shaohua Liu |
Journal | Oral oncology
(Oral Oncol)
Vol. 47
Issue 1
Pg. 27-32
(Jan 2011)
ISSN: 1879-0593 [Electronic] England |
PMID | 21111670
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- ID1 protein, human
- Inhibitor of Differentiation Protein 1
- Vascular Endothelial Growth Factor C
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Topics |
- Animals
- Carcinoma, Squamous Cell
(genetics, metabolism)
- Cell Line, Tumor
- Down-Regulation
- Gene Silencing
(physiology)
- Humans
- Inhibitor of Differentiation Protein 1
(genetics, metabolism)
- Lymphangiogenesis
(genetics)
- Lymphatic Metastasis
- Mice
- Mice, Nude
- Mouth Neoplasms
(genetics, metabolism)
- Vascular Endothelial Growth Factor C
(genetics, metabolism)
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