Abstract | BACKGROUND: METHODS: Immunohistochemistry was applied to detect HBO1 protein expression in breast cancer specimens (n=112). The expression of protein level was scored by integral optical density (IOD) for further statistical analyses using SPSS. Real-time PCR was used to simultaneously measure mRNA levels of HBO1. The HBO1 protein expression in breast cancer cells was confirmed by western blot. RESULTS: HBO1 was highly expressed in breast cancer tissues and significantly correlated with estrogen receptor α (ERα) (p<0.001) and progestational hormone (PR) (p=0.002). HBO1 protein level also correlated positively with histology grade in ERα positive tumors (p=0.016) rather than ERα negative tumors. 17β-estradiol (E2) could upregulate HBO1 gene expression which was significantly inhibited by ICI 182,780 or ERα RNAi. E2-increased HBO1 protein expression was significantly suppressed by treatment with inhibitor of MEK1/2 ( U0126) in T47 D and MCF-7 cells. CONCLUSIONS: HBO1 was an important downstream molecule of ERα, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.
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Authors | Wen-zhong Wang, Hai-ou Liu, Yi-hong Wu, Yi Hong, Jun-wu Yang, Ye-heng Liu, Wei-bin Wu, Lei Zhou, Lin-lin Sun, Jie-jie Xu, Xiao-jing Yun, Jian-xin Gu |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 29
Pg. 140
(Nov 01 2010)
ISSN: 1756-9966 [Electronic] England |
PMID | 21040551
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Estrogen Receptor alpha
- Receptors, Progesterone
- Estradiol
- Histone Acetyltransferases
- KAT7 protein, human
- MAPK1 protein, human
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
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Topics |
- Blotting, Western
- Breast Neoplasms
(genetics, metabolism)
- Cell Line, Tumor
- Estradiol
(genetics, metabolism)
- Estrogen Receptor alpha
(genetics, metabolism)
- Female
- Gene Expression
- Gene Expression Regulation, Neoplastic
- Histone Acetyltransferases
(biosynthesis, genetics)
- Humans
- Immunohistochemistry
- Mitogen-Activated Protein Kinase 1
(genetics, metabolism)
- Mitogen-Activated Protein Kinase 3
(genetics, metabolism)
- Receptors, Progesterone
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
(physiology)
- Transfection
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