[Effect of sustained release of recombinant rat insulin-like growth factor-1 from poly (lactide-CO-glycolide ) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes].

Abstract

OBJECTIVE:

To evaluate the effect of sustained release of recombinant rat insulin-like growth factor-1(rrIGF-1) from poly (lactide-CO-glycolide) (PLGA) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes.

METHODS:

Type 2 diabetes models were successfully established in 20 male Goto-Kakizaki rats, which were then randomly divided into treatment group (sustained release of rrIGF-1 from PLGA microspheres were loaded on the peri-implant areas, n=10) and diabetic group (loaded with isodose placebo from PLGA microspheres, n=10). Another ten male SD rats served as control group (did not sustain any loading). Titanium implants were inserted into the tibias of 30 diabetic and normal animals. Four, 5, and 8 weeks after implantation, local blood samples around the implants were obtained for the determination of serum osteocalcin (OCN), serum bone specific alkaline phosphatase (B-ALP), and serum procollagen I carboxyterminal propeptide (PICP) with enzyme linked immunosorbent assays.

RESULTS:

Four weeks after implantation, OCN, B-ALP, and PICP were significantly lower in both treatment group and diabetic group than in control group(both P<0.05). Five weeks after implantation, serum OCN and B-ALP levels of the diabetic group were significantly lower than those of the other two groups (all P<0.05). Serum PICP levels of both diabetic group and treatment group were significantly lower than that of control group(both P<0.05). The OCN level in the trealment group was significantly higher in the post-operative 5th week than in the post-operative 4th week, while the PICP levels in the diabetic group were significantly lower than those in the treatment group and control group in the post-operative 8th week (both P<0.05).

CONCLUSION:

Sustained release of rrIGF-1 from PLGA microspheres loaded on the local peri-implant areas can promote bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes.

• Authors: Miao Yu, Xin-Min Hui, Feng Wang, Ning Zhang, He-Peng Zhang, Ying-Liang Song
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 32 Issue 3 Pg. 260-4 (Jun 2010) ISSN: 1000-503X [Print] China
• PMID: 20602874 (Publication Type: Journal Article)

Chemical References

• Delayed-Action Preparations
• Dental Implants
• insulin-like growth factor-1, rat
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid
• Insulin-Like Growth Factor I

Topics

• Animals
• Delayed-Action Preparations
• Dental Implants
• Diabetes Mellitus, Experimental (physiopathology)
• Diabetes Mellitus, Type 2 (physiopathology)
• Disease Models, Animal
• Implants, Experimental
• Insulin-Like Growth Factor I (administration & dosage, pharmacology)
• Lactic Acid (administration & dosage, pharmacology)
• Male
• Microspheres
• Osteogenesis (drug effects)
• Polyglycolic Acid (administration & dosage, pharmacology)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Rats
• Rats, Inbred Strains