Abstract | BACKGROUND: METHODS: The effect of Iripallidal on proliferation and apoptosis in glioma cell lines was evaluated by MTS, colony formation and caspase-3 activity. The effect of iripallidal to regulate (i) Akt/mTOR and STAT3 signaling (ii) molecules associated with cell cycle and DNA damage was evaluated by Western blot analysis. The effect of Iripallidal on telomerase activity was also determined. RESULTS:
Iripallidal (i) induced apoptosis, (ii) inhibited Akt/mTOR and STAT3 signaling, (iii) altered molecules associated with cell cycle and DNA damage, (iv) inhibited telomerase activity and colony forming efficiency of glioma cells. In addition, Iripallidal displayed anti-proliferative activity against non- glioma cancer cell lines of diverse origin. CONCLUSION: The ability of Iripallidal to serve as a dual-inhibitor of Akt/mTOR and STAT3 signaling warrants further investigation into its role as a therapeutic strategy against GBM.
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Authors | Nitin Koul, Vivek Sharma, Deobrat Dixit, Sadashib Ghosh, Ellora Sen |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 328
(Jun 24 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20576128
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 26-hydroxy-22-methylcycloirid-16-enal
- Cyclohexanols
- Acrolein
- Proto-Oncogene Proteins c-akt
- TOR Serine-Threonine Kinases
- Telomerase
- Caspase 3
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Topics |
- Acrolein
(analogs & derivatives, pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Brain Neoplasms
(drug therapy, metabolism, pathology)
- Caspase 3
(metabolism)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cyclohexanols
(pharmacology)
- Glioblastoma
(drug therapy, metabolism, pathology)
- Humans
- Proto-Oncogene Proteins c-akt
(antagonists & inhibitors, metabolism)
- Signal Transduction
(drug effects)
- TOR Serine-Threonine Kinases
(antagonists & inhibitors, metabolism)
- Telomerase
(antagonists & inhibitors, metabolism)
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