Protective effects of recombinant human granulocyte macrophage colony stimulating factor on H1N1 influenza virus-induced pneumonia in mice.

Abstract

Protective effects of recombinant human granulocyte macrophage colony stimulating factor (rHuGM-CSF) on H1N1 influenza virus infection was studied in vivo and in vitro. Mice were infected with H1N1 influenza A viruses and rHuGM-CSF at doses of 0.34, 0.67, and 1.34mgkg(-1)d(-1) was administrated for 7days before the mice were infected with influenza virus and continued for a further 3days. Compared with control mice, rHuGM-CSF was demonstrated to increase the survival rate of the infected mice by 50.0%, 55.6%, and 80.0% and increased the mean survival days by 25.7%, 30.0%, and 46.8%, respectively. Histopathological study of the lungs in pneumonia mice found that pre-treatment with rHuGM-CSF significantly ameliorated lung injury induced by influenza virus infection. In vitro study demonstrated that when rHuGM-CSF were co-incubated with peripheral blood mononuclear cells (PBMCs), the PBMCs culture supernatant induced a dose-dependent reduction of virus-induced cytopathic effect (CPE) in Madin-Darby canine kidney (MDCK) cells in vitro. These results suggested that rHuGM-CSF might be an effective and potential protection for H1N1 influenza virus-induced pneumonia.

• Authors: Hai Huang, Hong Li, Pei Zhou, Dianwen Ju
• Journal: Cytokine (Cytokine) Vol. 51 Issue 2 Pg. 151-7 (Aug 2010) ISSN: 1096-0023 [Electronic] England
• PMID: 20427198 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Recombinant Proteins
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Animals
• Female
• Granulocyte-Macrophage Colony-Stimulating Factor (therapeutic use)
• Humans
• Influenza A Virus, H1N1 Subtype
• Leukocytes, Mononuclear (virology)
• Lung (pathology, virology)
• Male
• Mice
• Mice, Inbred BALB C
• Orthomyxoviridae Infections (drug therapy, mortality, pathology)
• Pneumonia, Viral (drug therapy, mortality, pathology)
• Recombinant Proteins