[Effects of Shenkangwan on renal expressions of angiotensin II and its type I receptor in rats with early diabetic nephropathy].

Abstract

OBJECTIVE:

To investigate the effects of Shenkangwan on the expressions of angiotensin II (AngII) and its type I receptor (AT(1)R) and the renalprotection mechanism of Shenkangwan in rats with early diabetic nephropathy (DN).

METHODS:

The rat models of DN established by a single injection of streptozotocin were randomly divided into 4 groups, namely the model group, Shenkangwan treatment group, irbesartan treatment group, and Shenkangwan and irbesartan treatment group, with normal rats as the control. All the rats received daily gavage for 8 weeks. The urinary protein quality in 24 h and plasma and renal contents of AngII were measured. The expressions of AT1R at the protein and mRNA levels in the kidney tissues were measured by immunohistochemistry and reverse transcription-polymerase chain reaction, respectively. The pathological changes of the kidney were observed microscopically.

RESULTS:

In DN rats, Shenkangwan reduced the urinary protein quantity in 24 h and the contents of AngII in the plasma and kidney tissues, decreased the renal expressions of AT(1)R protein and mRNA, and alleviated the morphological damage of the kidney.

CONCLUSIONS:

Shenkangwan offers renalprotection against DN probably by reducing the contents of AngII in the plasma and kidney tissues and inhibiting renal AT(1)R expressions.

• Authors: Hai-bo Long, Hong-xin Niu, Xiao-yun Li, Zhao-zhong Xu, Hui Zhang, Juan Zhong, Lian-bo Wei
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 30 Issue 4 Pg. 805-9 (Apr 2010) ISSN: 1673-4254 [Print] China
• PMID: 20423855 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiotensin II Type 1 Receptor Blockers
• Drugs, Chinese Herbal
• RNA, Messenger
• Receptor, Angiotensin, Type 1
• shenkangwan
• Angiotensin II

Topics

• Angiotensin II (genetics, metabolism)
• Angiotensin II Type 1 Receptor Blockers (therapeutic use)
• Animals
• Diabetic Nephropathies (drug therapy, metabolism)
• Drugs, Chinese Herbal (therapeutic use)
• Kidney (metabolism)
• Male
• Phytotherapy
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptor, Angiotensin, Type 1 (genetics, metabolism)