Phosphatase enzymes. Cytochemical study of pleomorphic adenoma and normal human salivary glands.

Abstract

Human parotid glands, submandibular glands, and pleomorphic adenomas were examined by electron microscopic histochemistry. All epithelial cells of the normal salivary glands showed plasma membrane adenosine triphosphatase (ATPase) and inosine diphosphatase (IDPase) activity. However, myoepithelial cells reacted most intensely. Pleomorphic adenomas showed epithelial cells within solid and ductal portions of the tumors that were variably reactive for both ATPase and IDPase. Histochemical examination of the epithelial cells in the myxoid portions of the tumors did not provide conclusive evidence as to the nature of their progenitor cells. Surface-associated phosphatases (alkaline phosphatase, ATPase, and IDPase) cannot be reliably used as histochemical markers of salivary gland myoepithelial cells. Therefore, morphological and phosphatase histochemical studies that intend to examine the role of myoepithelial cells in salivary gland neoplasms must be interpreted with care.

• Authors: D J Innes Jr, L S Cutler
• Journal: Archives of pathology & laboratory medicine (Arch Pathol Lab Med) Vol. 102 Issue 2 Pg. 90-4 (Feb 1978) ISSN: 0003-9985 [Print] United States
• PMID: 203248 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Inosine Nucleotides
• Phosphoric Monoester Hydrolases
• Acid Anhydride Hydrolases
• Adenosine Triphosphatases
• inosine diphosphatase
• Magnesium

Topics

• Acid Anhydride Hydrolases
• Adenoma, Pleomorphic (enzymology, pathology, ultrastructure)
• Adenosine Triphosphatases (analysis)
• Histocytochemistry
• Humans
• Inosine Nucleotides
• Magnesium (pharmacology)
• Microscopy, Electron
• Parotid Neoplasms (enzymology, pathology, ultrastructure)
• Phosphoric Monoester Hydrolases (analysis)
• Submandibular Gland