Abstract | BACKGROUND AIMS: METHODS: The heparanase epitope was modified by ER retrieval signal, and dendritic cells (DC) were pulsed with the modified peptide. The location and presentation of the modified peptide were detected, and the potential of the anti- tumor response was assessed. RESULTS: The modified peptide could target the ER of DC to form stable major histocompatibility complex (MHC)- peptide complexes. In addition, vaccination with DC pulsed with the modified peptide elicited a robust, specific CTL response, significantly inhibited tumor growth and prolonged the lifespan of the mice. CONCLUSIONS:
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Authors | Kun Zhou, Huaping Zhu, Liang Huang, Yanhong Guo, Yiqun Yan |
Journal | Cytotherapy
(Cytotherapy)
Vol. 12
Issue 6
Pg. 735-42
(Oct 2010)
ISSN: 1477-2566 [Electronic] England |
PMID | 20230227
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cancer Vaccines
- Epitopes, T-Lymphocyte
- Peptide Fragments
- Protein Sorting Signals
- Heparin Lyase
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Topics |
- Animals
- Antigen Presentation
- Cancer Vaccines
- Cell Growth Processes
- Cell Line
- Cytotoxicity, Immunologic
- Dendritic Cells
(cytology, immunology, metabolism, transplantation)
- Endoplasmic Reticulum
(metabolism)
- Epitopes, T-Lymphocyte
(metabolism)
- Heparin Lyase
(chemical synthesis, genetics)
- Immunization
- Melanoma, Experimental
(immunology, pathology, therapy)
- Mice
- Mice, Inbred C57BL
- Peptide Fragments
(chemical synthesis, genetics, metabolism)
- Protein Sorting Signals
(genetics)
- Skin Neoplasms
(immunology, pathology, therapy)
- T-Lymphocytes, Cytotoxic
(immunology, metabolism, pathology)
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