Abstract | OBJECTIVE: METHODS: Three groups of study subjects were recruited: (1) 97 HIV negative healthy donors, (2) 92 HIV patients of A1 to A3 stages and (3) 146 HIV patients of B1 to C3 stages. Total RNA was extracted from PBL. Reverse transcription (RT)-PCR and quantification PCR were developed for the SDF-1alpha transcriptional study. R1 value was calculated based on the ratio of SDF-1alpha copies to beta-globin copies. RESULTS:
SDF-1alpha transcription is heterogeneous among the three study groups. The SDF-1alpha transcription was significantly up-regulated during late stage of HIV infection than the healthy donors. Correlation analysis indicated that R1 value was negatively correlated to CD4+ T cells counts (P = 0.002); and positively correlated to virus load (P = 0.001). The result demonstrated an association between SDF-1alpha transcription and disease progression. CONCLUSION:
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Authors | Xiu-Ying Zhao, Rui-Shan Li, Jia-Qing Huang, Zhi-Wei Chen, Bo-Jian Zheng |
Journal | Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
(Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi)
Vol. 23
Issue 3
Pg. 204-7
(Jun 2009)
ISSN: 1003-9279 [Print] China |
PMID | 20104779
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Case-Control Studies
- Cells, Cultured
- Chemokine CXCL12
(genetics, metabolism)
- HIV Infections
(genetics, metabolism, virology)
- HIV-1
(genetics, physiology)
- Humans
- Lymphocytes
(metabolism, virology)
- Transcription, Genetic
- Up-Regulation
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