Abstract |
We have previously constructed a recombined vascular basement membrane derived multifunctional peptide ( rVBMDMP) which can inhibit tumor growth. The aim of this study is to explore the effects and mechanisms of rVBMDMP on growth and motility/invasion in human A549 lung carcinoma cells. The effect of rVBMDMP on A549 cell viability was determined by MTT assay while the motility/invasion was measured by scratch and transwell assays. Molecules that responded to rVBMDMP treatment of A549 cells were explored using the high-throughput Cancer Pathway Finder cDNA Microarray. We identified 16 genes that were up-regulated, including GZMA, ITG alphaV, MCAM and Kiss1 and 21 genes that were down-regulated, including uPA, uPAR, CDC25A, IGF1 and FGF2. Selective differentially expressed genes were further analyzed by real-time quantitative PCR and Western blot analysis. These findings contribute to the understanding of the molecular mechanisms mediating rVBMDMP action, and suggest that rVBMDMP is a promising novel agent for the treatment of human lung carcinoma.
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Authors | Cheng-kun Wang, Jian-guo Cao, Bo Peng, Yi-xue Gu, Guo-pei Zheng, Zhi-min He |
Journal | Cancer letters
(Cancer Lett)
Vol. 292
Issue 2
Pg. 261-8
(Jun 28 2010)
ISSN: 1872-7980 [Electronic] Ireland |
PMID | 20053497
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Peptides
- Recombinant Proteins
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Topics |
- Basement Membrane
(chemistry)
- Blotting, Western
- Cell Division
(drug effects)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Humans
- Immunoprecipitation
- Lung Neoplasms
(pathology)
- Neoplasm Invasiveness
- Peptides
(pharmacology)
- Polymerase Chain Reaction
- Recombinant Proteins
(pharmacology)
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