Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: We measured H5-neutralization antibody titers pre- and post-vaccination using the H5N1 micro-neutralization test (MN) and H5pp tests in subjects given seasonal vaccines and in selected sera from European elderly volunteers in a H5N1 vaccine trial who had detectable pre-vaccination H5N1 MN antibody titers. We found detectable (titer > or = 20) H5N1 neutralizing antibodies in a minority of pre-seasonal vaccine sera and evidence of a serological response to H5N1 in others after seasonal influenza vaccination. There was excellent correlation in the antibody titers between the H5N1 MN and H5pp tests. Similar correlations were found between MN and H5pp in the pre- vaccine sera from the cohort of H5N1 vaccine trial recipients. CONCLUSIONS/SIGNIFICANCE: Heterosubtype neutralizing antibody to H5N1 in healthy volunteers unexposed to H5N1 is mediated by cross-reaction to the H5 haemagglutinin.
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Authors | Jean-Michel Garcia, Stephanie Pepin, Nadège Lagarde, Edward S K Ma, Frederick R Vogel, Kwok H Chan, Susan S S Chiu, J S M Peiris |
Journal | PloS one
(PLoS One)
Vol. 4
Issue 11
Pg. e7918
(Nov 20 2009)
ISSN: 1932-6203 [Electronic] United States |
PMID | 19936250
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Hemagglutinins
- Influenza Vaccines
- Neuraminidase
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Topics |
- Aged
- Animals
- Antibodies, Viral
(chemistry)
- Cell Line
- Child
- Dogs
- Europe
- Hemagglutinins
(chemistry)
- Humans
- Influenza A Virus, H5N1 Subtype
(immunology)
- Influenza Vaccines
(immunology)
- Influenza, Human
(blood, immunology, virology)
- Middle Aged
- Neuraminidase
(metabolism)
- Neutralization Tests
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