Matrix metalloproteinase 1 promoter polymorphisms and risk of myocardial infarction: a case-control study in a Spanish population.

Abstract

OBJECTIVES:

Inherited and acquired risk factors contribute to the development of the atherosclerotic lesion and its most common clinical manifestation, myocardial infarction (MI). Multiple studies have suggested a role for matrix metalloproteinases (MMPs) in atherosclerosis, and several functional polymorphisms in the MMP-1 gene have been linked to the risk of MI. The aim of this study was to evaluate the association between MMP-1 promoter polymorphisms and early MI in a Spanish cohort.

METHODS:

We carried out a case-control study with 261 unrelated patients who had suffered an MI before 55 years of age and 194 healthy controls, all male and smokers. The genotypes for the three MMP-1 promoter polymorphisms -1607 1G/2G, -519 A/G, and -340 T/C were determined through PCR-restriction fragment length polymorphism. Allelic, genotypic, and haplotypic frequencies were statistically compared between groups.

RESULTS:

Frequencies of the three polymorphisms did not differ between patients and controls. The -1607 1G/2G and -519 A/G variants were in linkage disequilibrium. Analysis of the haplotype frequencies showed significant associations of the 2G(-1607)-G(-519)-T(-340) (odds ratio = 2.40; 95% confidence interval = 1.27-4.55; P<0.006) and 1G(-1607)-G(-519)-T(-340) (odds ratio = 0.68; 95% confidence interval = 0.50-0.94; P<0.05) haplotypes with the risk of early MI.

CONCLUSION:

MMP-1 promoter polymorphisms are associated with the risk of early MI in a Spanish population of smoking males.

• Authors: Pablo Román-García, Eliecer Coto, Julián R Reguero, Jorge B Cannata-Andía, Iñigo Lozano, Pablo Avanzas, César Morís, Isabel Rodríguez
• Journal: Coronary artery disease (Coron Artery Dis) Vol. 20 Issue 6 Pg. 383-6 (Sep 2009) ISSN: 1473-5830 [Electronic] England
• PMID: 19620856 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MMP1 protein, human
• Matrix Metalloproteinase 1

Topics

• Adult
• Case-Control Studies
• Gene Frequency
• Genetic Predisposition to Disease
• Haplotypes
• Humans
• Linkage Disequilibrium
• Male
• Matrix Metalloproteinase 1 (genetics)
• Middle Aged
• Myocardial Infarction (enzymology, genetics)
• Odds Ratio
• Polymorphism, Genetic
• Promoter Regions, Genetic
• Risk Assessment
• Risk Factors
• Sex Factors
• Smoking (adverse effects)
• Spain