PET of EGFR antibody distribution in head and neck squamous cell carcinoma models.

Abstract

UNLABELLED:

Epidermal growth factor receptor (EGFR) is a well-characterized protooncogene that has been shown to promote tumor progression in solid cancers. Clinical results for EGFR targeting with specific monoclonal antibodies (mAbs) such as cetuximab and panitumumab are promising; however, most studies indicate that only a subgroup of patients receiving the mAbs benefit from the immunotherapy, independent of EGFR expression level. To understand the in vivo kinetics of antibody delivery and localization, we performed small-animal PET studies with (64)Cu-labeled panitumumab in xenografts derived from 3 cell lines of human head and neck squamous cell carcinoma (HNSCC).

METHODS:

Nude mice bearing HNSCC tumors with different levels of EGFR expression were imaged with small-animal PET using (64)Cu-1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)-panitumumab. Antibody distribution in the tumors was confirmed by ex vivo immunostaining using panitumumab and fluorescein 5(6)-isothiocyanate (FITC) panitumumab. CD31 immunostaining and Evans blue assay were also performed to assess the tumor vascular density and permeability.

RESULTS:

Among these 3 tumor models, UM-SCC-22B tumors with the lowest EGFR protein expression showed the highest (64)Cu-DOTA-panitumumab accumulation, whereas SQB20 tumors with the highest EGFR expression showed the lowest (64)Cu-DOTA-panitumumab accumulation. Ex vivo staining demonstrated that SQB20 cells still had extremely high EGFR expression after forming tumors in nude mice, indicating that the low uptake of (64)Cu-DOTA-panitumumab in SQB20 tumors was not due to the loss of EGFR expression. The results from CD31 immunostaining and Evans blue permeability assay suggest that the low vessel density, poor vascular permeability, and binding site barrier are likely responsible for the overall low tumor uptake of the highly EGFR-expressing SQB20 tumors.

CONCLUSION:

The results from this study provide a possible explanation for the lack of an observed correlation between therapeutic efficacy of cetuximab and panitumumab and EGFR expression level as determined by immunohistochemistry or fluorescent in situ hybridization and may shed new light on the complications of anti-EGFR mAb therapy for HNSCC and other malignancies.

• Authors: Gang Niu, Zibo Li, Jin Xie, Quynh-Thu Le, Xiaoyuan Chen
• Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 50 Issue 7 Pg. 1116-23 (Jul 2009) ISSN: 0161-5505 [Print] United States
• PMID: 19525473 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antineoplastic Agents
• Copper Radioisotopes
• Radiopharmaceuticals
• Panitumumab
• ErbB Receptors

Topics

• Animals
• Antibodies, Monoclonal (immunology, pharmacokinetics)
• Antineoplastic Agents (pharmacokinetics)
• Carcinoma, Squamous Cell (diagnostic imaging, metabolism)
• Cell Line, Tumor
• Copper Radioisotopes (pharmacokinetics)
• Disease Models, Animal
• ErbB Receptors (immunology, metabolism)
• Female
• Head and Neck Neoplasms (diagnostic imaging, metabolism)
• Humans
• Mice
• Mice, Nude
• Panitumumab
• Positron-Emission Tomography (methods)
• Radiopharmaceuticals (pharmacokinetics)
• Tissue Distribution