Abstract |
2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD) is a ubiquitously distributed endocrine-disrupting chemical and reproductive toxicant. In order to elucidate low-dose TCDD-mediated effects on reproductive or endocrine functions, female Sprague-Dawley rats were orally administered various concentrations (20, 50, or 125 ng/kg once weekly) TCDD for 29 wk. A proteomic analysis of the ovaries by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry showed distinct changes in the levels of several proteins that are relevant markers of TCDD toxicity. Serum estradiol (E2) levels of TCDD-treated animals were markedly lower than control. There were no significant differences in bone mineral density (BMD) of femurs. The body weight of the 125-ng/kg TCDD group was significantly decreased relative to control and there was also a significant reduction in absolute and relative ovarian weights. Expressions of selenium binding protein 2, glutathione S-transferase mu type 3, Lrpap1 protein, NADPH, and peptidylprolyl isomerase D were upregulated, while prohibitin and N-ethylmaleimide-sensitive factor expression levels were downregulated. Data provide further insight into the mechanisms by which TCDD disrupts ovarian function by indicating which differential protein expressions following low-dose TCDD exposure.
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Authors | Xi Chen, Xiao-ming Ma, Shi-wei Ma, Pieter-Jan Coenraads, Chun-mei Zhang, Jing Liu, Li-jun Zhao, Min Sun, Nai-jun Tang |
Journal | Journal of toxicology and environmental health. Part A
(J Toxicol Environ Health A)
Vol. 72
Issue 11-12
Pg. 717-26
( 2009)
ISSN: 1528-7394 [Print] England |
PMID | 19492234
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Environmental Pollutants
- Isoenzymes
- LDL-Receptor Related Protein-Associated Protein
- Polychlorinated Dibenzodioxins
- Prohibitins
- Repressor Proteins
- Selenbp1 protein, rat
- Selenium-Binding Proteins
- Estradiol
- NADP
- Glutathione Transferase
- glutathione transferase M3-3
- N-Ethylmaleimide-Sensitive Proteins
- Peptidylprolyl Isomerase
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Topics |
- Administration, Oral
- Animals
- Down-Regulation
- Environmental Pollutants
(administration & dosage, toxicity)
- Estradiol
(metabolism)
- Female
- Glutathione Transferase
(metabolism)
- Isoenzymes
(metabolism)
- LDL-Receptor Related Protein-Associated Protein
- N-Ethylmaleimide-Sensitive Proteins
(metabolism)
- NADP
(metabolism)
- Organ Size
(drug effects)
- Ovary
(drug effects, metabolism)
- Peptidylprolyl Isomerase
(metabolism)
- Polychlorinated Dibenzodioxins
(administration & dosage, toxicity)
- Prohibitins
- Proteomics
- Rats
- Rats, Sprague-Dawley
- Repressor Proteins
(metabolism)
- Selenium-Binding Proteins
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Up-Regulation
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