Anti-tumor activity of oxypeucedanin from Ostericum koreanum against human prostate carcinoma DU145 cells.

Abstract

PURPOSE:

Oxypeucedanin has been reported to have various biological activities. We investigated the efficacy of a coumarin compound, oxypeucedanin, from Ostericum koreanum against the human prostate carcinoma cell line DU145.

MATERIAL AND METHODS:

Oxypeucedanin (C(16)H(14)O(5), mw: 286) was isolated through silica gel chromatography and characterized by NMR. The cells were treated with oxypeucedanin (25, 50, and 100 microM) for 24-72 hours, and cell growth and death were then assayed. The cell cycle progression and apoptotic effects were also assessed by western blotting.

RESULTS:

Treatment with oxypeucedanin inhibited cell growth and induced cell death in DU145 cells. Furthermore, oxypeucedanin-induced cell growth inhibition was associated with an increase in G2-M arrest in cell cycle progression in DU145 cells in a dose and time-dependent manner. G2-M arrest by oxypeucedanin was associated with decreased levels of cyclin A, cyclin B1, Cdc2, and pCdc2. Oxypeucedanin-induced cell death was associated with significant increases in apoptosis and cleaved caspase-3 and poly-(ADP-ribose) polymerase.

CONCLUSION:

These finding suggest a novel anticancer effect for oxypeucedanin, mediated via induction of G2-M cell cycle arrest and apoptosis in human prostate carcinoma DU145 cells.

• Authors: Tae Jin Kang, Sook Yeon Lee, Rana P Singh, Rajesh Agarwal, Dong Sool Yim
• Journal: Acta oncologica (Stockholm, Sweden) (Acta Oncol) Vol. 48 Issue 6 Pg. 895-900 ( 2009) ISSN: 1651-226X [Electronic] England
• PMID: 19322700 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Cyclins
• Furocoumarins
• Poly(ADP-ribose) Polymerases
• Caspase 3
• oxypeucadanin

Topics

• Antineoplastic Agents (isolation & purification, pharmacology)
• Apiaceae (chemistry)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Division (drug effects)
• Cell Proliferation (drug effects)
• Cyclins (metabolism)
• Furocoumarins (isolation & purification, pharmacology)
• G2 Phase (drug effects)
• Humans
• Immunoblotting
• Male
• Plant Roots (chemistry)
• Poly(ADP-ribose) Polymerases (metabolism)
• Prostatic Neoplasms (metabolism, pathology)
• Tumor Cells, Cultured