Quantitative analysis of phosphatidylcholines and phosphatidylethanolamines in urine of patients with breast cancer by nanoflow liquid chromatography/tandem mass spectrometry.

Abstract

Phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) from urine of patients with breast cancer were qualitatively and quantitatively analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC-ESI-MS-MS). Urinary phospholipids (PLs) were extracted from three different categories of patients (non-cancer controls and breast cancer patients before and after surgery) by first lyophilizing only 1 mL of urine sample to enrich PLs. Next, nLC-ESI-MS-MS analysis of intact urinary phospholipids was performed, resulting in structural identification of 21 PCs and 12 PEs, followed by quantitative analysis using a multiple standard addition method. This study demonstrated that nLC-ESI-MS-MS can be powerfully utilized for the study of relative changes in the contents and concentration of urinary PCs and PEs from breast cancer patients: total concentration of PCs and PEs of patient sample increased to (144 +/- 9)% and (171 +/- 11)%, respectively, compared to control sample but they decreased significantly following surgery.

• Authors: Hanna Kim, Hye Kyeong Min, Gu Kong, Myeong Hee Moon
• Journal: Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 393 Issue 6-7 Pg. 1649-56 (Mar 2009) ISSN: 1618-2650 [Electronic] Germany
• PMID: 19194696 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphatidylcholines
• Phosphatidylethanolamines

Topics

• Adult
• Breast Neoplasms (urine)
• Chromatography, Liquid (methods)
• Female
• Humans
• Middle Aged
• Phosphatidylcholines (urine)
• Phosphatidylethanolamines (urine)
• Spectrometry, Mass, Electrospray Ionization (methods)
• Tandem Mass Spectrometry (methods)
• Time Factors