Abstract | PURPOSE: EXPERIMENTAL DESIGN: Ten patients were vaccinated twice with a set of 10 overlapping p53-SLP in a phase I/II trial. Both the safety and the breadth, magnitude, and polarization of vaccine-induced p53-specific T cells was evaluated in blood samples drawn before and after vaccination by IFN-gamma enzyme-linked immunospot, proliferation, cytokine secretion, and multiparameter flow cytometry. The migratory capacity of p53-specific T cells was evaluated by assessing their presence in a biopsy of the second vaccination site. RESULTS: Toxicity was limited to grade 1/2, mostly at the vaccination site. p53-specific T-cell responses were induced in 9 of 10 colorectal cancer patients as measured by IFN-gamma enzyme-linked immunospot, proliferation, and cytokine bead array. In 6 of 9 tested patients, p53-specific T-cell reactivity persisted at least 6 months. Furthermore, p53-specific T cells isolated from the vaccination site were characterized as CD4+ T cells producing both T-helper types 1 and 2 cytokines on stimulation with p53 peptide and p53 protein. Multiparameter flow cytometry revealed that only a minor population of the p53-specific CD4+ T cells was optimally polarized. CONCLUSIONS: The p53-SLP vaccine is safe and capable to induce p53-specific T-cell responses in patients treated for colorectal cancer. New trials should focus on improving the polarization of the p53-SLP vaccine-induced T-cell response.
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Authors | Frank M Speetjens, Peter J K Kuppen, Marij J P Welters, Farah Essahsah, Anne Marie E G Voet van den Brink, M Graziella Kallenberg Lantrua, A Rob P M Valentijn, Jaap Oostendorp, Lorraine M Fathers, Hans W Nijman, Jan W Drijfhout, Cornelis J H van de Velde, Cornelis J M Melief, Sjoerd H van der Burg |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 15
Issue 3
Pg. 1086-95
(Feb 01 2009)
ISSN: 1078-0432 [Print] United States |
PMID | 19188184
(Publication Type: Clinical Trial, Phase I, Clinical Trial, Phase II, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cancer Vaccines
- Cytokines
- Tumor Suppressor Protein p53
- Vaccines, Subunit
- p53 synthetic long peptide vaccine
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Topics |
- Aged
- CD4-Positive T-Lymphocytes
(immunology)
- Cancer Vaccines
(adverse effects, immunology, therapeutic use)
- Colorectal Neoplasms
(immunology, pathology, therapy)
- Cytokines
(biosynthesis)
- Female
- Humans
- Male
- Middle Aged
- Neoplasm Metastasis
- T-Lymphocytes, Cytotoxic
(immunology)
- Tumor Suppressor Protein p53
(adverse effects, immunology, therapeutic use)
- Vaccines, Subunit
(adverse effects, immunology, therapeutic use)
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