Plasma advanced glycation endproduct, methylglyoxal-derived hydroimidazolone is elevated in young, complication-free patients with Type 1 diabetes.

Abstract

OBJECTIVES:

Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-free young individuals with Type 1 diabetes (T1DM). The relationship of plasma free MG-H to hemoglobin A1C (A1C) and plasma methylglyoxal levels was also determined.

DESIGN AND METHODS:

A solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed, and free plasma MG-H levels were measured in 40 T1DM patients (DM group), aged 6-21 years, and 11 non-diabetics (ND group), 6-22 years. Methylglyoxal was measured using LC-MS/MS and A1C by a Tosoh G7 high-performance liquid chromatograph.

RESULTS:

Our method showed high recovery, sensitivity and short run-time. Plasma free MG-H (nmol/L) was higher (p<0.001) in the DM group (1318+/-569; mean+/-standard deviation) as compared to the ND group (583+/-419). Within the DM group, plasma free MG-H did not correlate with plasma methylglyoxal or A1C.

CONCLUSIONS:

Our LC-MS/MS method to measure free MG-H in plasma may be useful for future clinical application. The increased levels of free MG-H observed in individuals with TIDM are not merely the result of short term changes in glucose or methylglyoxal, but may reflect long-term alterations to tissue proteins.

• Authors: Yingchun Han, Edward Randell, Sudesh Vasdev, Vicki Gill, Matthew Curran, Leigh Anne Newhook, Marie Grant, Donna Hagerty, Celine Schneider
• Journal: Clinical biochemistry (Clin Biochem) Vol. 42 Issue 7-8 Pg. 562-9 (May 2009) ISSN: 1873-2933 [Electronic] United States
• PMID: 19154730 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycation End Products, Advanced
• Imidazoles
• imidazolone

Topics

• Adolescent
• Child
• Chromatography, Liquid
• Diabetes Complications
• Diabetes Mellitus, Type 1 (blood)
• Glycation End Products, Advanced (blood)
• Humans
• Imidazoles (blood)
• Molecular Structure
• Tandem Mass Spectrometry
• Young Adult