Regulation of the leucocyte chemoattractant receptor FPR in glioblastoma cells by cell differentiation.

Abstract

The G protein-coupled formylpeptide receptor (FPR), known to mediate phagocytic leucocyte chemotaxis in response to bacterial- and host-derived agonists, was expressed by tumor cells in specimens of surgically removed more highly malignant human gliomas. In human glioblastoma cell lines, FPR activation increased cell motility, tumorigenicity and production of angiogenic factors. In studies of the mechanistic basis for the selective expression of FPR in more highly malignant gliomas, we found that the DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (Aza), while promoting the differentiation of human glioblastoma cells, downregulated FPR expression. Aza also reduced the global methylation levels in glioblastoma cells and activated the pathway of p53 tumor suppressor. Methylation-specific polymerase chain reaction revealed that Aza treatment of tumor cells reduced the methylation of p53 promoter, which was accompanied by increased expression of p53 gene and protein. In addition, overexpression of p53 in glioblastoma cells mimicked the effect of Aza treatment as shown by increased cell differentiation but reduction in FPR expression, the capacity of tumor sphere formation in soft agar and tumorigenesis in nude mice. Furthermore, Aza treatment or overexpression of the wild-type p53 in glioblastoma cells increased the binding of p53 to FPR promoter region shown by chromatin immunoprecipitation. These results indicate that increased methylation of p53 gene retains human glioblastoma cells at a more poorly differentiated phase associated with the aberrant expression of FPR as a tumor-promoting cell surface receptor.

• Authors: Jian Huang, Keqiang Chen, Jiaqiang Huang, Wanghua Gong, Nancy M Dunlop, O M Zack Howard, Xiuwu Bian, Yuqi Gao, Ji Ming Wang
• Journal: Carcinogenesis (Carcinogenesis) Vol. 30 Issue 2 Pg. 348-55 (Feb 2009) ISSN: 1460-2180 [Electronic] England
• PMID: 19037090 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Formyl Peptide
• TP53 protein, human
• Tumor Suppressor Protein p53
• Decitabine
• DNA Modification Methylases
• Azacitidine

Topics

• Animals
• Azacitidine (analogs & derivatives, pharmacology)
• Brain Neoplasms (metabolism, pathology)
• Cell Differentiation
• Cell Line, Tumor
• Cell Proliferation
• Chemotaxis
• DNA Modification Methylases (antagonists & inhibitors)
• Decitabine
• Gene Expression Regulation, Neoplastic
• Glioblastoma (metabolism, pathology)
• Humans
• Methylation
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Promoter Regions, Genetic
• Receptors, Formyl Peptide (biosynthesis)
• Transplantation, Heterologous
• Tumor Suppressor Protein p53 (metabolism)