Abstract |
Enhancer of zeste homolog 2 (EZH2) is a mammalian histone methyltransferase that contributes to the epigenetic silencing of target genes and regulates the survival and metastasis of cancer cells. EZH2 is overexpressed in aggressive solid tumors by mechanisms that remain unclear. Here we show that the expression and function of EZH2 in cancer cell lines are inhibited by microRNA-101 (miR-101). Analysis of human prostate tumors revealed that miR-101 expression decreases during cancer progression, paralleling an increase in EZH2 expression. One or both of the two genomic loci encoding miR-101 were somatically lost in 37.5% of clinically localized prostate cancer cells (6 of 16) and 66.7% of metastatic disease cells (22 of 33). We propose that the genomic loss of miR-101 in cancer leads to overexpression of EZH2 and concomitant dysregulation of epigenetic pathways, resulting in cancer progression.
|
Authors | Sooryanarayana Varambally, Qi Cao, Ram-Shankar Mani, Sunita Shankar, Xiaosong Wang, Bushra Ateeq, Bharathi Laxman, Xuhong Cao, Xiaojun Jing, Kalpana Ramnarayanan, J Chad Brenner, Jindan Yu, Jung H Kim, Bo Han, Patrick Tan, Chandan Kumar-Sinha, Robert J Lonigro, Nallasivam Palanisamy, Christopher A Maher, Arul M Chinnaiyan |
Journal | Science (New York, N.Y.)
(Science)
Vol. 322
Issue 5908
Pg. 1695-9
(Dec 12 2008)
ISSN: 1095-9203 [Electronic] United States |
PMID | 19008416
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- 3' Untranslated Regions
- DNA-Binding Proteins
- Histones
- MIRN101 microRNA, human
- MicroRNAs
- RNA, Small Interfering
- Transcription Factors
- EZH2 protein, human
- Enhancer of Zeste Homolog 2 Protein
- Polycomb Repressive Complex 2
- Lysine
|
Topics |
- 3' Untranslated Regions
- Algorithms
- Breast Neoplasms
(genetics, metabolism)
- Cell Line, Tumor
- DNA-Binding Proteins
(genetics, metabolism)
- Disease Progression
- Enhancer of Zeste Homolog 2 Protein
- Epigenesis, Genetic
- Female
- Gene Expression Regulation, Neoplastic
- Genome, Human
- Histones
(metabolism)
- Humans
- Lysine
(metabolism)
- Male
- Methylation
- MicroRNAs
(genetics, metabolism)
- Neoplasm Metastasis
- Neoplasms
(genetics, metabolism)
- Polycomb Repressive Complex 2
- Promoter Regions, Genetic
- Prostatic Neoplasms
(genetics, metabolism, pathology)
- RNA, Small Interfering
(genetics)
- Stomach Neoplasms
(genetics, metabolism)
- Transcription Factors
(genetics, metabolism)
|