Abstract | BACKGROUND: RESULTS: Cytoplasmic HuR staining was found in the epithelium of 10% of normal mucosa, 14.3% of adenomas and 88.9% of adenocarcinomas from FAP patients (p < 0.01) and in 68.8% of sporadic colorectal carcinomas. High epithelial COX-2 immunostaining was observed in 10% of normal, 8% of adenomas and all adenocarcinomas from FAP patients (p < 0.01) and in 69.5% of sporadic colorectal carcinomas. Positive cytoplasmic HuR immunostaining correlated with high COX-2 immunoreactivity in colon mucosa of FAP patients (p < 0.01) and in sporadic colorectal carcinomas. (p = 0.016) MATERIALS AND METHODS: HuR and COX-2 protein expression were studied by immunohistochemistry of normal colon mucosa (N=20), adenomas (N=112), carcinomas (N=9) from patients with FAP, and 141 sporadic colorectal adenocarcinomas (Dukes B and C). CONCLUSIONS: HuR is increasingly expressed in the cytoplasmic epithelial compartment in consecutive stages of the adenoma- carcinoma sequence in FAP. Also, COX-2 levels correlate with cytoplasmic expression of HuR in colonic epithelium of FAP patients and in sporadic colorectal cancer specimens. The role of cytoplasmic expression of HuR as a biomarker for progression of adenomas in FAP needs further study.
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Authors | Lodewijk A A Brosens, Josbert J Keller, Leena Pohjola, Caj Haglund, Folkert H Morsink, Christine Iacobuzio-Donahue, Michael Goggins, Francis M Giardiello, Ari Ristimäki, G Johan A Offerhaus |
Journal | Cancer biology & therapy
(Cancer Biol Ther)
Vol. 7
Issue 3
Pg. 424-7
(Mar 2008)
ISSN: 1555-8576 [Electronic] United States |
PMID | 18094611
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Surface
- ELAV Proteins
- ELAV-Like Protein 1
- ELAVL1 protein, human
- Phosphoproteins
- RNA-Binding Proteins
- Cyclooxygenase 2
- PTGS2 protein, human
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Topics |
- Adenomatous Polyposis Coli
(enzymology, genetics)
- Antigens, Surface
(genetics)
- Colonic Neoplasms
(enzymology, genetics)
- Cyclooxygenase 2
(genetics)
- Cytoplasm
(physiology)
- ELAV Proteins
- ELAV-Like Protein 1
- Epithelial Cells
(enzymology, pathology)
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Humans
- Intestinal Mucosa
(pathology)
- Phosphoproteins
(metabolism)
- RNA-Binding Proteins
(genetics)
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