Abstract | BACKGROUND & AIMS: METHODS: The physical and functional interaction between Topo IIalpha and the beta-catenin/TCF-4 nuclear complex was evaluated by immunoprecipitation, immunofluorescence microscopy, 2-hybrid assay, and luciferase reporter assay. RESULTS: CONCLUSIONS:
Topo IIalpha interacts with beta-catenin as a novel transcriptional co-activator. A new drug targeting the interaction of Topo IIalpha with beta-catenin as well as its catalytic activity might be more effective for suppressing aberrant Wnt signaling and proliferation of colorectal cancer cells than the current Topo II inhibitors.
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Authors | Lin Huang, Miki Shitashige, Reiko Satow, Kazufumi Honda, Masaya Ono, Jisoo Yun, Akihiro Tomida, Takashi Tsuruo, Setsuo Hirohashi, Tesshi Yamada |
Journal | Gastroenterology
(Gastroenterology)
Vol. 133
Issue 5
Pg. 1569-78
(Nov 2007)
ISSN: 1528-0012 [Electronic] United States |
PMID | 17983804
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- DNA-Binding Proteins
- Enzyme Inhibitors
- RNA, Small Interfering
- TCF Transcription Factors
- TCF7L2 protein, human
- Thiobarbiturates
- Topoisomerase II Inhibitors
- Transcription Factor 7-Like 2 Protein
- Wnt Proteins
- beta Catenin
- Etoposide
- DNA Topoisomerases, Type II
- merbarone
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Topics |
- Adenoma
(genetics, metabolism, pathology)
- Antigens, Neoplasm
(genetics, metabolism)
- Cell Line, Tumor
- Cell Proliferation
- Colorectal Neoplasms
(genetics, metabolism, pathology)
- DNA Topoisomerases, Type II
(genetics, metabolism)
- DNA-Binding Proteins
(antagonists & inhibitors, genetics, metabolism)
- Enzyme Inhibitors
(pharmacology)
- Etoposide
(pharmacology)
- Gene Expression Regulation, Neoplastic
- Humans
- RNA, Small Interfering
(pharmacology)
- Signal Transduction
(physiology)
- TCF Transcription Factors
(genetics, metabolism)
- Thiobarbiturates
(pharmacology)
- Topoisomerase II Inhibitors
- Transcription Factor 7-Like 2 Protein
- Wnt Proteins
(physiology)
- beta Catenin
(genetics, metabolism)
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