Human bullous pemphigoid antigen 2 transgenic skin elicits specific IgG in wild-type mice.

Abstract

Bullous pemphigoid antigen 2 (BPAG2) is targeted by autoantibodies in patients with bullous pemphigoid (BP), and absent in patients with one type of epidermolysis bullosa (OMIM #226650). A keratin 14 promoter construct was used to produce transgenic (Tg) mice appropriately expressing human BPAG2 (hBPAG2) in murine epidermal basement membrane (BM). Grafts of Tg skin placed on gender-matched, syngeneic wild type (Wt) or major histocompatibility complex I (MHC I)-/- mice elicited IgG that bound human epidermal BM and BPAG2. Production of such IgG in grafted mice was prompt (detectable within 16+/-2 days), robust (titer > or = 1,280), durable (present > or = 380 days), and correlated with the involution and loss of Tg skin grafts. MHC II-/- mice grafted with Tg skin did not develop anti-hBPAG2 IgG or graft loss indicating that MHC II:CD4+ T cell interactions were crucial for these responses. Tg skin grafts on Wt mice developed neutrophil-rich infiltrates, dermal edema, subepidermal blisters, and deposits of immunoreactants in epidermal BM. This model shows fidelity to alterations seen in patients with BP, has relevance to immune responses that may arise in patients with epidermolysis bullosa following BPAG2 gene replacement, and can be used to identify interventions that may block production of IgG against proteins in epidermal BM.

• Authors: Edit B Olasz, Jooyoung Roh, Carole L Yee, Ken Arita, Masashi Akiyama, Hiroshi Shimizu, Jonathan C Vogel, Kim B Yancey
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 127 Issue 12 Pg. 2807-17 (Dec 2007) ISSN: 1523-1747 [Electronic] United States
• PMID: 17657247 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Autoantibodies
• Autoantigens
• Immunoglobulin G
• Non-Fibrillar Collagens

Topics

• Animals
• Autoantibodies (chemistry)
• Autoantigens (genetics, physiology)
• CD4-Positive T-Lymphocytes (metabolism)
• Humans
• Immune System
• Immunoglobulin G (metabolism)
• Immunohistochemistry
• Keratinocytes (metabolism)
• Kinetics
• Mice
• Mice, Transgenic
• Models, Biological
• Non-Fibrillar Collagens (genetics, physiology)
• Pemphigoid, Bullous (genetics, immunology)
• Transfection
• Collagen Type XVII