Cytotoxicity of purified cassava linamarin to a selected cancer cell lines.

Abstract

Cassava (Manihot esculenta Crantz) is a known source of linamarin, but difficulties associated with its isolation have prevented it from being exploited as a major source. A batch adsorption process using activated carbon proved successful in its isolation, with ultrafiltration playing a pivotal role in its purification. Thirty-two minutes of contact time was required for 60 g of extract, yielding 1.7 g of purified product. Picrate paper, infra-red and 'HNMR analysis confirmed the presence and structure of linamarin. Cytotoxic effects of linamarin on MCF-7, HT-29 and HL60 cells were determined using the MTT assay. Cytotoxic effects were significantly increased in the presence of linamarase (P-glucosidase), with a 10-fold decrease in the IC50 values obtained for HL-60 cells. This study thus describes a method for the isolation and purification of linamarin from cassava, as well as its cytotoxicity potential.

• Authors: Christopher Avwoghokoghene Idibie, Hajierah Davids, Sunny Esayegbemu Iyuke
• Journal: Bioprocess and biosystems engineering (Bioprocess Biosyst Eng) Vol. 30 Issue 4 Pg. 261-9 (Jul 2007) ISSN: 1615-7591 [Print] Germany
• PMID: 17566787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Nitriles
• Plant Extracts
• linamarin

Topics

• Antineoplastic Agents (administration & dosage)
• Apoptosis (drug effects)
• Cell Line, Tumor (drug effects, physiology)
• Cell Survival (drug effects)
• Humans
• Manihot (chemistry)
• Nitriles (administration & dosage)
• Phytotherapy (methods)
• Plant Extracts (administration & dosage)
• Seeds (chemistry)