Abstract |
Nordy is a synthesized chrial compound. To investigate the effects of nordy (25 - 100 micromol x L(-1)) on the function of formylpeptide receptor (FPR) of malignant human glioma cells, human glioblastoma cell line U87 was used to detect its proliferation, migration, calcium mobilization, vascular endothelial growth factor ( VEGF) mRNA and protein levels after activation of FPR by its agonist N-formyl-methionyl-leucyl-phenylalanine (fMLF). Cell proliferation, migration ability, VEGF mRNA, VEGF protein and calcium mobilization were evaluated by cell counting, chemotaxis assay, RT-PCR, ELISA and spectrometry. Nordy (50 - 100 micromol x L(-1)) potently inhibited the proliferation, migration and calcium mobilization of U87 cells induced by fMLF (P < 0.05). Moreover, 100 micromol x L(-1) nordy showed a significantly impaired VEGF mRNA expression and protein secretion induced by fMLF (P < 0.05). Nordy could inhibit FPR functioning in glioma cell proliferation, migration and angiogenesis, which might be a possible mechanism of its anti- cancer effects.
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Authors | Jian-Hong Chen, Xiu-Wu Bian, Xiao-Hong Yao, Shi-Xin Yang, Chang-Rong Xu, Xiang-Dong Zhou, Yi-Fang Ping |
Journal | Yao xue xue bao = Acta pharmaceutica Sinica
(Yao Xue Xue Bao)
Vol. 42
Issue 3
Pg. 257-62
(Mar 2007)
ISSN: 0513-4870 [Print] China |
PMID | 17520823
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- RNA, Messenger
- Receptors, Formyl Peptide
- Vascular Endothelial Growth Factor A
- nordy
- N-Formylmethionine Leucyl-Phenylalanine
- Masoprocol
- Calcium
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Topics |
- Antineoplastic Agents
(pharmacology)
- Calcium
(metabolism)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Glioblastoma
(genetics, metabolism, pathology)
- Humans
- Masoprocol
(analogs & derivatives, pharmacology)
- N-Formylmethionine Leucyl-Phenylalanine
(pharmacology)
- RNA, Messenger
(biosynthesis, genetics)
- Receptors, Formyl Peptide
(agonists, metabolism, physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Spectrophotometry
(methods)
- Vascular Endothelial Growth Factor A
(biosynthesis, genetics)
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