Interaction of cholera enterotoxin with cultured adrenal tumor cells.

Abstract

In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was preincubated with the toxin or added to adrenal cells 10 min before CT. In the preincubation studies a molar ratio of Gm1 to toxin of 3:1 was necessary for half-maximal inhibition of steroidogenesis. On the other hand, horse serum anticholeragenoid neutralized the steroidogenic response to cell-bound CT by 50% if it was added to adrenal monolayer cultures 15 min after the toxin. Specific antiserum was able to neutralized 20% of the toxin-induced activity even if it was added to adrenal cultures 2 h after CT. Phase contrast microscopy demonstrated that partial neutralization of the biochemical effect of CT by horse serum anticholeragenoid was accompanied by partial prevention of toxin-induced rounding of adrenal cells. Further studies showed that pretreatment of cultured adrenal cells with a maximal dose of CT increased cyclic adenosine 3'-5'-monophosphate formation in response to a maximal stimulating dose of adrenocorticotropin. This result suggested potentiation of hormonal activation of adenylate cyclase in intact adrenal tumor cells in response to CT.

• Authors: R M Wishnow, E Lifrak, C Chen
• Journal: Infection and immunity (Infect Immun) Vol. 12 Issue 4 Pg. 768-71 (Oct 1975) ISSN: 0019-9567 [Print] United States
• PMID: 172445 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antitoxins
• Enterotoxins
• Gangliosides
• Adrenocorticotropic Hormone
• Cyclic AMP

Topics

• Adrenal Gland Neoplasms (metabolism)
• Adrenocorticotropic Hormone (pharmacology)
• Animals
• Antitoxins
• Cholera
• Cyclic AMP (analysis)
• Drug Synergism
• Enterotoxins (metabolism, pharmacology)
• Gangliosides (pharmacology)
• Horses
• In Vitro Techniques
• Neoplasms, Experimental (metabolism)
• Neutralization Tests